| Infrared spectroscopy technology has many advantages such as highly sample analysis speed, no chemical pollution, instrument operation and maintenance are simple. It has a wide range of applications in chemical industry, medicine, agriculture, environment protection, biology, medical science and other various fields.Near infrared spectroscopy combined with chemometrics methods could realize the qualitative or quantity analysis of different substances. Mid-IR spectral technology in the application of chemical biology sensor is still in its infancy, expected in the following two aspects will become a new hotspot: First, Looking for heterozygous membrane materials that can effectively enrich and fixed polarity organic substances and biological molecules, for new chemical and biological sensors construction.These materials must be easily elution on CaF2, crystalline materials KBr, ZnSe, Ge, et al that could be easily used in the infrared instruments, further more the materials must not interfere with the response that caused by chemical biology molecular recognition ;second, developing a the new method of infrared-based nano markers for biological sensors. Nano-tag method has universally recognized and wide used application in fluorescence, electrochemical, radiation immunobiology analysis etc, it is a kind of effective method of signal enhancement. Mid-IR nano-material preparation and application research is still very rare reports in the literature, but it is undoubtedly a promising new method of biological analysis. To sum up, membrane and nano-materials in biological sensing application combined with infrared spectroscopy will play a more important role in biochemical analysis and surface science.This paper mainly does the following research.(1)In the second chapter, we applied near infrared spectral analysis technology combined with the mobile window partial least-square mode discriminant analysis method for the discriminant analysis of the six nestle milk powder, 1-6 months of different brands baby milk powder, south different stages of dried milk and the soy milk powder. Judging from the result, the used method could reject the independent and useless information that interference with classification, this can more effectively processing the nonlinear and the complex linear correlation, thus this method has certain directive significance to realize the online quality monitoring and tracking the sources of the raw materials.(2)The application of miscellaneous membrane in sensor are obtaining more and more attention .In the third chapter we have synthesized by sol-gel method polystyrene/titanium dioxide organic and inorganic heterozygous membrane, and its chemical composition and distribution shape were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, infrared imaging and contact Angle. From the result, we can calculate that this membrane can be easily enriched on and stripped off the CaF2 crystal, this indicate that the membrane is suitable for the infrared biosensor.(3)Nanomaterials with so many excellent functions such as it are very sensitive to the external environment, temperature, humidity, light etc. Nanoparticles fixed on the biological macromolecules can be made into the sensor used for biological signal detection and signal conversion and magnification. In the forth chapter ,Precipitation reforming process was used to synthesized CuSCN nanoparticles, sodium hexametaphosphate was used as inorganic dispersant, the obtained products were used in infrared biosensor constructed. |
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