The Study On Anammox Process Of Sewage Treatment Technology Of Ecology Of Microorganisms

Molecular biology technology has been developing rapidly in recent years, especiallyin the environmental nitrogen cycle and nitrogen pollution prevention and control researchhas made significant development. In the Nitrogen pollution prevention, especially in thebiological nitrogen removal of municipal wastewater, researchers have been paying muchattention to the removal of ammonia. In the nitrification-anaerobic ammonium oxidationdenitrification process, anaerobic ammonium oxidation bacteria(AnAOB) plays a vitalrole to removal of total nitrogen in waste water. In the stage of nitrification, parts of NH4+is inverted to NO2-in the reactor, then NH4+and NO2-which is as electron acceptor bothenter the stage of anaerobic ammonia oxidation and are invert to N2. While AnAOB canuse the energy that is generate in this chemical reaction for its metabolism. This paperanalyze, research and identify the microbial community structure of anaerobic granularsludge in anaerobic ammonium oxidation system by the two molecular biological methodsof PLFA map analysis and16S rDNA sequence homology analysis. Thus provide systemoperating parameters, operating status and the adjustment of treatment effect sometheoretical basis.By amplifying the total DNA that is from the bacteria of activated sludge inanammox reactor by16S rDNA universal primer and Planctomycetes-specific16S primer,cloning the DNA gene fragment and analyzing gene sequence homology. The formerresult indicate that, there has a high diversity of microbial communities in the anammoxsystem. After digesting the160clones by restriction endonuclease, they belong to31OTUs. The sequencing result show that all OTUs belong to7groups which areproteobacteria, bacteroides,Nitrospira,Acidobacteria,Chloroflexi, Candidate division OP10and Planctomycetes. The later result that there are43clones that are from50clonesbelong to Candidatus Kuenenia of Planctomycetes. It suggests that AnAOB account for86%in planctomycetes. It is true that AnAOB sequence can not be amplified by universalprimers, for so far, there is no AnAOB16S rDNA sequence that matches the CandidatusKuenenia sequence.In the anammox reactor, we also analyzed the bacteria in which there have An-hzogene fragment. The result is consistent with the above, most of the sequences that has theAn-hzo gene belong to Kuenenia stuttgartiensis of Candidatus Kuenenia. Finally, we dynamically analyzed the microbial community of anaerobic granularsludge in different time of the anammox reactor by method of PLFA. In all of the PLFAsthat were extracted in the samples mainly divide into odd-chain saturated fatty acids,even-chain saturated fatty acids, monounsaturated fatty acids, branched-chain fatty acids,polyunsaturated fatty acids and some cyclopropane fatty acids. At different times,microbial community structure of anaerobic granular activated sludge in anammox reactorhas no significant difference. In the anammox process, Microorganisms mainly includebacteria, most of which are anaerobic bacteria.Anaerobic bacteria are the dominant florabacteria in the system. While the changes of PLFA content of anaerobic bacteria atdifferent times affect the removal of NO2-and NH4+, so anaerobic bacteria is the key to thesmooth running of the reactor.