Fabrication Of Micropatterned TiO₂Nanotube And Research The Biocompatibilityprimary

We used a photolithographic approach and electrochemical anodization technology to produce micropatterned TiO2nanotube layers surrounded by compact oxide ontitanium surface. The TiO2nanotube arrays were characterized by fourier transform infrared spectrometer(FTIR) and scanning electron microscope (SEM). The results showed that HF/H3PO4electrolyte had a strong corroding effect on photoresistand bond with Ti surface, though part of photoresist adhered to the surface of sample. To improve the graphs architecture, The HF/H3PO4electrolyte was replaced by glycerin anodic oxidation liquid. From the results of FTIR and SEM, the photoresist could be removed clearly. Due to the large viscosity of glycerol system electrolyte, it was unstable to prepare smaller size (10/5μ m) nanotube arrays, and only some of nanotubes was found at the section where the normal large-size nanotubes should form. Thus the following researches mainly focused on larger size graphics (20/10μm) nanotube arrays. Through the analysis of hydrolysis tentacles and XRD, the graphical titania nanotubes treated at500℃in air for3hours obtained better hydrophilicity, and the rutile and anatase mixed crystalline structure maintain antithrombogenicity and biological activity, laying the groundwork for the following cell culture.The results of platelet adhesion experiment showed that micropatterned-nanotube sample reduced the platelet adhesion, which was better than nanotube sample and Ti sample.The results of ECs culture experiment showed that at the preliminary stage micropatterned-nanotube guided the ECs orientation and promoted the proliferation of ECs. The results of SMCs adhesion experiment showed that micropatterned-nanotube guided the SMCs oriented growth and the amount of SMCs on the surface of micropatterned-nanotube sample was less than the Ti sample. Due to the combined action of two structures, it would then be possible to restrain the platelet and SMCs simultaneously, which may be a new method of surface modification for blood-contact materials.