The Study On Invivo Anti-Oxidation And Factors Influence Squalene Accumulation For Strain Produced Squalene

Thraustochytrids can biosynthesize not only high amount of DHA, but also high content of squalene as well. This study used a high-yeild squalene in thraustochytrid genus strains, and the strains of systematic classification of molecular biology and eventually to establish it as Aurantiochytrium sp. Genus, named BR-MP4-A1. The in vivo effects of squalene/sterols on lipid peroxidation in Thraustochytrium sp, as well as squalene, fatty acid metabolic pathways studied.1. The strains of systematic classification of molecular biology and eventually to establish it as Aurantiochytrium sp. Genus, named BR-MP4-A1.2. To investigate anti-oxidation for cells in vivo. H2O2, cumeme hydroperoxide (CHP), t-butyl hydroperoxide (t-BuOOH) and methyl jasmonate were used as inducer of lipid peroxidation, and lipid peroxidation was characterized by measuring malondialdehyde (MDA), and the survival rate by plate counting. None of the tested chemicals can induce lipid peroxidation in the current experiments, the inducers with high concentration lead to the cell death. The results may indicate that the accumulation of squalene or PUFA has the effect of anti-oxidation and protect the cells from damaging outside.3. According to the screening strain growth characteristics and squalene, unsaturated fatty acids, cholesterol derived from such studies established cerulenin, Terbinafine and Zaragozic acid in Thraustochytrium metabolic regulation of intracellular sites, cerulenin and Terbinafine could promoted the accumulation of squalene.4. Citric could promote the content of squalene when being added at Ohr, and the rate of cholesterol synthesis was higher than before after P-Glycerophate was added being at Oh and malate stimulated fatty acid synthesis.