| Aromatic amines and their derivatives are widely used in industry includingproduction of dyes, agricultural chemical, polymers, rubbers and pharmaceuticals.Due to their high solubility in water, aromatic amines can easily permeate through soiland contaminate groundwater. Nowadays most of anilines are known to be highlymutagenic and carcinogens and to form adducts with proteins and DNA. Furthermore,it is well known that these compounds are carcinogens and have been implicated ininducing cancer of the bladder.In this study, a novel method for the determination of nitroanilines in hair dyesamples has been developed based on poly(methacrylicacid-co-ethylene glycoldimethacrylate) monolith microextraction (PMME) and high-performance liquidchromatography (HPLC) analysis. Four nitroanilines, p-nitroaniline (PNAL),m-nitroaniline (MNAL), o-nitroaniline (ONAL), and2,4-dinitroaniline (DNAL), arestudied as representatives. To obtain optimum extraction efficiency, severalexperimental parameters includingsample flowrate, sample volume, sample pH, andeluent flowrate have been investigated.The optimized mobile phase was methanol–water (70:30, v/v), the flow rate wasmaintained at0.6mL/min, the column temperature was set at30°C, and the UVabsorbance detection was set to254nm. In the poly(MAA-EGDMA) monolithmicroextraction procedure, the sample flow rate was set at0.2mL/min, samplevolume maintained at1.0mL, sample pH was4.0, and eluent flow rate was0.1mL/min.Under the optimal experimental conditions, the linear regression coefficients ofthe standard curves are greater than0.9990. The limits of detection for p-nitroaniline,m-nitroaniline, o-nitroaniline, and2,4-dinitroaniline are0.012,0.008,0.018and0.005mg/mL, respectively. The intraday and interday relative standard deviations are lessthan3.1and5.4%, respectively. The proposed method is simple, rapid, sensitive, and competent when used for the determination of nitroanilines in hair dye samples andthe accuracy is assessed through recovery experiments. |
PDF Full Text Request