Study Of Purification And Funciation Of Daidzein And Genistein Extracted From Soybean Paste

Soy sauce is one of the traditional beans fermented food in China.Mold secreted glucosidasethat hydrolyze Isoflavones glycosidic bonds to Daidzein、Gentstein、Glycitein, et al. during fer--mentation. An explosion of research have been shown that Isoflavones Fight tumour, prevent bo--ne loose, protect cardiovascular and relaxed women’s Menopause Syndrome.The study of Daidzein and Gentstein which extracted, isolated and purificated from Soy sau--ce,with a view toward testing antioxidant activities and anticancer activity. The results showedthat:1、This paper studied the extraction of Daidzein and Genistein in soybean paste by,ultrasoundmethod.Based on comprehensive consider--ation of extraction rate,economy and simplicity,theultrasonic extraction process of Daidzein and Genistein was optimized by response surfacemethod,the extraction parameters has been determined finally.The optimum extraction conditionsof Daidzein were:extracting time by ultrasonic was61minutes, extraction temperature was48℃,ethanol concentration was74%,the yield rate of Daidzein in soybean paste was484.610μg/g;The optimum extraction conditions of Genistein were:ethanol concentration was78%,extraction temperature was59min, extraction temperature was49℃,extraction power wasweak,the yield rate of Genistein in extracting solution was100.07μg/g.2、The isolation, purifying of Daidzein and Gentstein were studied.The results showed thatAB-8resin has a good isolation and purifying ability for Daidzein and Gentstein. And the adsor--ption elution conditions were: sample quantity was3mg, adsorption rate was2mL/min, the eluti--on rate was2mL/min.Under this conditions, the purity of Daidzein and Gentstein respectivelywas71.49%,64.78%. The SephadexLH-20dextran gel used in further respectively purification ofDaidzein and Gentstein. And the process conditions were: sample quantity was5mg, the elutionrate was2mL/min, ethanol concentration was70%.Elution peaks were measured by HPLC and thethird elution peak was Daidzein and Gentstein,and the purity of Daidzein and Gentstein respecti--veely was94.31%,90.72%.3、The anti-oxidation activity and anti tumor activity of Daidzein and Gentstein extract fromSoy sauce were studied. The Daidzein and Gentstein had strong reducing capacity,it was below itsrespectively standard reference substance. The Vc had better reducing capacity than Daidzein andit less than Gentstein. The DPPH free radical and hydroxyl radical scavenging ability of Daidzeinand Gentstein between Vc and respectively standard reference substance,and Vc is less thanstandard reference substance.Using MTT experiment determined the inhibition rate.The experi--mental results show that the Daidzein and Gentstein had inhibition on the growth of Humanbreast cancer cells, Daidzein was not good as Gentstein.