Preparation Of Functional Materials On Microfluidic Devices And Their Application In Analytical Chemistry

Microfluidic has been widely used in biochemical analysis and clinicaldiagnosis, due to the capability of high throughput, low consumption andfast detection.Microfluidic technology, which is being developed fastcurrently, is widely used in the sample analysis,due to its micro-scalechannels and flexible design. In this thesis uses a combination ofmicrofluidic chip and functional materials for rapid detection of therequired samples, in order to get the content of the composition has animportant role in complex samples. The time and chemical consumptionwas greatly reduced and simplified pretreatment aproach.The mian principles of microfluidic chip technology,the investigationand application of functional material combined with microfluidic chipsin the sample detection progress.we synthesize a simple method for fabricating a series of poly(ethyleneglycol) diacrylate (PEG-DA) hydrogel microstructures insidemicrofluidic channels as probe for proteins and glucose. And PEGmicrocolumns were used for the parallel detection of multiplecomponents. Using tetrabromophenol blue (TBPB) and the horseradish peroxidase/glucose oxidase reaction system, bovine serum albumin (BSA)and glucose in human urine were detected by color changes. The colorchanges for BSA within a concentration range of1–150lM, and glucosewithin a range of50mM–2M could be directly distinguished by eyes orprecisely identified by optical microscope. The detection process issimple just completed in5min only consume the sample5mL for aparallel measurement of blood glucose concentration and proteinconcentration in human urine. This method is easy to use, in the testingprocess does not require special readout equipment or power source wasrequired during the diagnosis process, which is promising for anapplication in rapid point-of-care diagnosis.A porous polymer monolithic column for solid-phase microextractionand chemiluminescence detection was integrated into a simplemicrofluidic chip for the extraction and determination of catechins ingreen tea. The porous polymer was prepared by poly (glycidylmethacrylate-co-ethylene dimethacrylate) and modified withethylenediamine. Catechins can be concentrated in the porous polymermonolithic column and react with potassium permanganate to givechemiluminescence. The microfluidic chip is reusable with highsensitivity and very low reagent consumption. The on-linepreconcentration and detection can be realized without an elution step.Microfluidic chip combination of porous polymer monolith complex sample pre-treatment to achieve a qualitative and quantitative analysis ofgreen tea polyphenols. With low sample consumptions (<1mL), shortdetection time (<5min) and good accuracy. The sample separation andon-line detection also demonstrated.