Study Of The Ecology Toxic Effects Of Single And Combined Pollutionbetween Cu And Sulfadiazine

With large and medium cities and towns of the rapid development of intensive livestock farmingsystems, it contains all kinds of poultry and animal feces of veterinary medicine antibiotics in outputincreases, and the resulting in a large animal waste eliminated from the body with veterinary medicine.Contain residues of organic manure as a veterinary drug into farmland can cause agricultural soil pollution,the health of human beings and soil ecological system to produce potential hazards. Cu is toxicenvironmental pollutants, along with the development of metallurgical industry, a large number of Cu intothe environment, Cu and veterinary medicine would cause the composite pollution. To evaluate theveterinary medicine and Cu single and composite pollution on the ecological environment caused by thepotential risks, the single and composite pollution of the commonly used veterinary medicine such assulfa-drugs pyramiding (SD) and Cu to soil and plant would be worked.Single and joint toxic effects of veterinary drugs [sulfadiazine](SD) and copper (Cu) on seedgermination, root elongation and shoot elongation of tomato, Chinese cabbage, cucumberand radish werecarried out. In the single-factor experiments, there was a dose-response relationship between theconcentration of SD or Cu and the inhibitory rate of crop root elongation and shoot elongation (p<0.05),but no correlation was found between the concentration of each pollutant and the inhibitory rate of cropseed germination (p>0.05). SD showed a much higher toxic effect on crops than did Cu. The IC50(halfinhibitation concentration) values of SD on root elongation of tomato, Chinese cabbage, cucumber andradish were33.64,1.8,581.5and3.822mg/kg, respectively, the corresponding values of Cu were723.5,273.6,493.7and203.9mg/kg, respectively. In the joint effect tests, the mixture of SD and Cu hadsynergistic effects on the four crops when root elongation was selected as the toxicological endpoint. At thelow concentration of Cu (100mg/kg), they had markedly significant (p<0.01) linear interrelationships basedon the regression analyses. However, the synergic effect on Chinese cabbage and cucumber was notsignificant with the increase of added Cu concentration (p>0.05).Joint toxicity of SD and Cu was moredependent on Cu. Using potting to study the impact of single and combined pollution between SD and Cu to soil enzymeactivity on the soil-soybean system.The results showed that cadmium could outstanding inhibited theactivity of phosphates and invertase,early in the trial SD also could outstanding inhibited the activity ofunease, catalane, phosphates, but in the late in the performance of weak activation，and SD plays the role ofInhibition inverts activity continued to the end of the experiment. When SD concentration was constant, thecombined treatment on the inhibition rate of phosphates activity with increasing concentration of cadmiumin larger and later in the test cycle, the various enzyme activity also show such circumstances, this may bedue to impact of enzyme activity by cadmium was large and persistent, the effects of SD on the enzymeactivity in the latter part of the experiment weaken because of their self concentration and microbesresistant。Physiological toxicity of Chinese cabbage seedlings showed that chlorophyll content was significantlylower than the control under the late stress of high concentration Sad(1mg·kg-1and10mg·kg-1). Thesignificant decrease of soluble protein (SP) content and SOD activity in Chinese cabbage seedling root wasdetected after5day exposure of SD, and dose-response relationship was found on5day, so they could beconsidered as biomarkers of stress by SD in soil. Under the stress of SD, POD activity in leaves increasedfirstly and then decreased, and POD activity in root was higher than in leaves. Chinese cabbage seedlingsand composite pollution by Cu and SD, SD show leading role; the high content of composite processing,the joint toxicity performance for synergy effect. Composite pollution, Chinese cabbage for the chlorophyllcontent, soluble protein content, peroxides superoxide dismutase (sod), the effect of influenced by SD.Chinese cabbage the toxic effects of each index with the increase of composite process content increased;In the high content level of composite process, its toxic effect more remarkable, Cu and SD compositepollution clearly enhance to the toxic effects of cabbage.