| Citreoviridin, a fungal poison, is a common contaminant to wide range kinds ofproducts, so it is in urgent demand to establish a rapid, sensitive, and specificimmunoassay method for citreoviridin assay. In this study, reaction aldehyde residueswere produced when hydroxyl groups on adjacent carbon atoms were oxidized bysodium periodate, so the product was suitable for coupling with amine, and then thecitreoviridin-bovine serum albumin conjugate (CIT-BSA) was prepared. Here,anti-citreoviridin polyclonal antibody was prepared by immunizing mice withCIT-BSA conjugate.The specificity and sensitivity of the polyclonal antibody was determined byindirect competitive ELISA, and the results showed that the IC50value of thepolyclonal antibody was0.56μg/mL and no cross-reaction was found between thisantibody and other toxins used in this experiment. The citreoviridin recovery rates bythis polyclonal antibody were calculated through artificially citreoviridincontaminated rice powder, and it was found that the recovery rates ranged from70.5±0.08%to94.7±0.09%for inter-assay, and77.5±0.04%to95.4±0.18%forintra-assay, which indicated that this polyclonal antibody could detect trace CIT fromthe tested samples. Consequently, this study provided a specific and sensitiveanti-citreoviridin polyclonal antibody, which made the detection of citreoviridin moreaccurate, instant, and easier. |
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