Research On Separation.purification And Vitro Antioxidant Activity Of Proteins From Artemia

In this article,the Artemia’s protein was divided into water dissolved protein, aciddissolved protein, alkaline dissolved protein and prolamin protein according to theirdissolving abilities. Their antioxidant activities were also investigated,and choose one of thebest antioxidant activities of these proteins. Central composite test optimize its extractionprocess.At last,water dissolved protein of Artemia was isolated and purified to obtain thewater dissolved protein components with best vitro antioxidant activity,and its molecularweight were determined.(1)There are4kinds proteins,which are water dissolved protein, alkaline dissolvedprotein, prolamin protein and acid dissolved protein from defatted powder of Artemia.Theresults show that the contents of water dissolved protein, alkaline dissolved protein, prolaminprotein and acid dissolved protein accounted for defatted powder25.03％、26.98％、1.83％、0.53％.(2)Research on these proteins’ vitro antioxidant activities,and determined hydroxylradical,superoxide anion radical, DPPH·radical and total reducing activity. The resultsshow that water dissolved protein is the best protein for the antioxidant activities.(3) The single test choose5factors,which include pretreatment temperature,liquidmaterial ratio,pH,temperature and time. Central composite test select the3factors which issignificant to optimal extraction from these factors. The optimal extraction technical conditionwas as follows,liquid material ratio7.5:1, temperature49.8℃, extraction time2.8h. Thedeviation of predicted and measured value is0.88%.(4)Water dissolved crude protein was mainly composed of4sub-fractions,namelypeak-1,peak-2, peak-3and peak-4by DEAE-Sepharose fast-flow column chromatographyand the vitro antioxidant activity of Peak-2is higher than the other3sub-fractions. Peak-2was mainly composed of3sub-fractions,namely peak2-1,peak2-2and peak2-3by SephadexG-100gel-permeation column chromatography, and the vitro antioxidant activity of peak2-1 is higher than the other2sub-fractiions.(5) Peak2-1was identified by SDS-PAGE and its molecular weight is82.47KD. The vitroantioxidant activity of Peak2-1is much higher than those of crude protein.