| To study the preparation process and method of quality control of compoundPraziquantel nanoemulsion.1.Drug,oils, surface active agents, surface active agent is mixed. The particle sizeand distribution are investigated.Using the nanoemulsion stability experiment indifferent temperatures and time and the centrifugal experiment with4000r/min,30min,the stability of compound Praziquantel nanoemulsion is determined.The best formulation of compound Praziquantel nanoemulsion is: w （Praziq-uante）=l1.20﹪, w（Fenbendazole）=0.04﹪, w（Ethyl acetate）=11.98﹪, w（N,N-Dim-ethylacetamide）=5.39﹪, w（ethyl alcohol）=29.17﹪, w（glacial acetic acid）=2.83﹪,w（Tween-802）=3.02%, w（DSMO）=19.97%and distilled water6.40%；2.The compound Praziquantel nanoemulsion is clear and transparent, and hasgood stability. And an average diameter of compound Praziquantel nanoemulsiondrops is11.2nm.There are to be smaller than15％when the diameter is less than9nm，to be smaller than2.5％when more than20nm and about82.5％when from9to20nm.Nanoemulsion that is made meets all requirements of liquid nano-materials.3.Safety evaluation of diclazuril nanoemulsion is maked.The safety of compoundPraziquantel nanoemulsion was evaluated by acute toxicity test.The results showedLD50 of compound Praziquantel nanoemulsion was649.01mg·kg-1,95%confidenceinterval of LD50 was560.09～752.04mg·kg-1.So compound Praziquantelnanoemulsion meets a standard of low toxicity drug. |
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