Influence Of Seasonal Climate Change On Anaerobic Fermentative Hydrogen Production

Hydrogen energy is an ideal alternative of trandational fossil fuels due to its environment friendly, carborn-free, as well as sustainable utilization. So hydrogen energy could help relieve the energy shortage and solve the environmantal problems. The anaerobic fermentative hydrogen production from sludge, using biomass as the substrate, is a new hydrogen producing process in recent years.This paper investigated the effect of seasonal climate change on the hydrogen production with sludge as the bacteria source, and glucose as the substrate. After two years reseach, this paper proved that seasonal climate change had a large influence on the H2yield. And the influence showed a periodical variety annually. The highest hydrogen production appeared in summer and fall, but the H2yield declined in spring and winter. The optimal climate periods for biohydrogen production were around the Solar Term9(Grain in Beard), Jun.6th to21st; the Solar Term16(the Autumn Equinox) and the Solar Term17(Cold Dew), Sep.23rd to Oct.23rd each year.This paper studied the morphology of the bacteria in the sludge during the fermentation progress by Gram staining and microscope. It is found that the appearance of capsular materials from the H2producing bacteria is the reason resulting in the termination of hydrogen fermentationThis paper also investigated the hydrogen production process with Clostridium perfringens ATCC13124(CP) as the bacteria source, and glucose as the substrate. The inoculation and the seasonal climate change had minor effect on the H2production by pure CP. The other non-hydrogen production anaerobic bacteria could inhibit the H2production ability of CP. The hydrogen production of CP related to the purity of the bacteria. The higher the bacteria purity is, the more hydrogen it produced. The most suitable growth temperature of CP was37℃, and much higher or lower temperature proved harmfully to the H2production.This paper also investigated the corresponding relationship between the local atmospheric temperature and the hydrogen production from sludge. It is found that the variety of the local atmospheric temperature could influence the bioactivity of the hydrogen production bacteria and its growth ratio with the other bacteria in the sludge, further influence the hydrogen production. When the local atmospheric temperature is near37℃, the sludge would be domesticated when it is in store, so it leads to a high hydrogen production yield. The annual periodical change of the local atmospheric temperature was the fundamental cause resulted in the annual periodical change of the hydrogen production.