The New Methods Of Health Laboratory Technology For Formaldehyde And Phenol And Phenylamine

Chapter1：The significance and headways trend of the health laboratory technologiesfor formaldehyde, phenol and aniline were introduced respectively. The basic principlesfor three determination methods of the health laboratory technologies for them were alsointroduced briefly in this topic.Chapter2: A novel catalytic resonance fluorescence method was proposed for thedetermination of trace formaldehyde based on the fact that formaldehyde can catalyzepotassium bromate oxidizing fluorescein in sulfuric acid medium, resulting in decreasingof the resonance fluorescenc intensity of fluorescein. In this study, the maximum resonancefluorescence wavelength of fluorescein was502nm. There is a good linear relationshipbetween the reduced intensity value of RF and HCHO concentration in the concentrationrange of0.0238～0.175μg mL-1under optimal conditions. The standard curve regressionequation is△F=469.71+12020ρ, and the LOD was7.15×10-3μg mL-1. This method is quickand simple with a little interference. It can be applied to the determination of trace formaldehyde inair of room and water samples of environment with satisfactory results. The RSD was less than2.47%and the recovery rates were96.5%～104.6%.Chapter3: A catalytic resonance fluorescence method for the determination of tracephenol in aqueous solution was proposed based on the catalytic effect of phenol on theoxidation of PY by potassium bromate in sulphuric acid medium. The maximum resonancefluorescence peak of PY was located at556nm, the emission fluorescence peak of reactionsolution was blue shifted to540nm after add potassium bromated. The detection limit andthe linear concentration range for phenol were0.026μg mL-1and0.050～1.25μg mL-1, respectively. The method is quick and simple with little interference. It can be applied tothe determination of trace phenol in water samples of environment with satisfactory results.The RSD was1.05%、0.37%, and the recovery rate was96.8%～103.2%.Chapter4: The fluorescence characteristics of PhOH and PhNH2in the presence ofβ-CD as an enhancer were studied. A novel fluorescence method for the simultaneousdetermination of trace phenol and phenylamine in environmental waste water wasproposed based on combination of the synchronous fluorescence technology and thedual-wavelength method. The samples without separation can direct simultaneousdetermination.The measurement of the synchronous fluorescence spectra was carried out withwavelength difference of△λ=60nm for synchronous scanning. There were the linearrelationship between the synchronization fluorescence intensity difference (by thedual-wavelength method) and the concentration of PhOH or PhNH2in solution usingβ-CD as an enhancer. When PhOH was determinated, wavelengths328nm and364nmwere respectively selected as the measurement wavelength and the reference wavelengthunder the optimized experimental conditions. There is a good linear relationship betweenthe synchronous fluorescence intensity difference of the solution with the concentration ofPhOH in concentration range of0.015～4.0μg mL-1, and the detection limit was4.5ngmL-1. When PhNH2was mensurated, the determination wavelength and referencewavelength were346nm and307nm, respectively. The concentration range of the linearrelationship between the synchronous fluorescence intensity difference and theconcentration of PhNH2was0.0085～4.0μg mL-1. The detection limit was2.6ng mL-1.The method is simple and has the high accuracy, precision and the low detection limit. Themethod has been applied to determine the contents of PhOH and PhNH2in environmentalwater samples with the recoveries of99.0%～106.7%. The RSD were less than0.94%and1.18%, respectively. The sensitizing mechanism of β-CD was preliminarly discused.