Studies On Effect Of Pseudomonas Aeruginosa On The Production Of Lignocellulase From Phanerochaete Chrysosporium

Biosurfactant, as an environmentally friendly natural surfactant, is usually usedin the enzyme production process to promote microbial degradation of lignocellulose.However, the preparation process of biosurfactant is cockamamie, and the cost ofproduction is expensive, become bottlenecks of its widely used in industry. Becauseof this, explore directly added biosurfactant producing bacteria has an importantimpact on lignocellulose processing. This study examined in liquid conditions thegrowth and the generation of surfactant simultaneously add biosurfactant producingbacteria and white rot fungus, and also studied in solid conditions the effects ofPseudomonas aeruginosa (P.aeruginosa) on the production of ligin peroxidase,manganese peroxidase and CMCase from Phanerochaete chrysosporium(P.chrysosporium), in order to research the characteristics of the microbialdegradation of lignocellulose in the role of Pseudomonas aeruginosa.In this study, we selected two different types of P.aeruginosa, AB93066andATCC9027respectively. By measuring the surface tension of the fermentation brothof the two P. aeruginosa cultured for72hours, can be drawn that the two bacteria havestrong ability of producing biosurfactant. And used the rice straw as the carbon source,simultaneously added P.chrysosporium and P.aeruginosa in the liquid conditions,measured the changing conditions of surface tension and the absorbance of thallus ofthe fermentation broth at different times. The results showed that the surface tensionof the fermentation broth from P.aeruginosa ATCC9027was lower than that fromP.aeruginosa AB93066, and maintained a low level throughout the wholefermentation process. This showed that during the fermentation process, the bacteriacould produce biosurfactants, and could play a role in the whole fermentation process,laid the foundation for further study.The effects of P.aeruginosa on the production of ligin peroxidase, manganeseperoxidase and CMCase from P.chrysosporium in a solid substrate fermentationprocess were studied. Effects of different concentrations of P.aeruginosa on otherparameters, such as pH, surface tension of crude enzyme liquid, volatile organicmatter concentration were also evaluated. The results showed that P.aeruginosaAB93066at5.72%increased the production of LiP, MnP and CMCase by67.8%,57.9%and17.8%, respectively.While the P.aeruginosa ATCC9027at2.86%increasedthe production of LiP, MnP and CMCase by86.3%,49.3%and43.7%, respectively. The surface tension of crude enzyme liquid from P.aeruginosa ATCC9027was lowerthan that from P.aeruginosa AB93066. The addion of P.aeruginosa had no significantinfluence on the pH value. The variety of volatile organic matter concentration waspositively correlated with the changes of enzymatic activities.