Study On Extraction Of Protein From Millet Bran And Its Protective Effects On Liver Injury

Millet bran is a mixture that millet is excepted seed coat、aleurone layer and germ, in the rice milling process. China is the origin of millet cultivation, and wide planting area, the annual output is very large, which about40tons of millet bran. Currently, the study compared the rice bran protein is more then millet bran protein,that has not been reported, so the development of millet bran protein products have a practical significance. In this study, the defatted millet bran protein as a raw material, mainly of millet bran albumin, and hydrolyzed protein extract and the albumin purification, molecular weight and amino acid analysis, of millet bran protein and hydrolyzed protein chemistry through animal testing and alcoholic liver injury in rats, the functional properties of millet bran protein is a preliminary study.1. The extracted from millet bran polysaccharide:The hot water extraction method to extract the millet bran polysaccharide, the optimum extraction conditions:80°C, the solid-liquid ratio1:20, extraction5h, pH3, the optimum process conditions polysaccharide extracted extraction rate of2.63%.2. The extracte of millet bran protein:The main research are millet bran albumin and protein hydrolyzate, the optimal extraction conditions of millet bran albumin extracted is pH9.0,55℃, extracted twice with extraction of2h and3h respectively, in the extraction rate of47.34%in optimal conditions; millet bran protein hydrolyzate of the optimal extraction conditions is the enzyme dosage1500U/g, pH8.5,38℃, and extraction of7h, the extraction rate of38.42%in optimal conditions, the finalmillet bran protein extraction rate can reach to85.76%.3. The purification and analysis of millet bran albumin:millet bran protein extract obtained contains more reducing sugars and ash impurities, that can through fixed yeast fermentation to remove the reducing sugar,and through ultrafiltration to remove ash and other impurities.The results of millet bran albumin purity to73.6%, sugar content reduced to1.12%, ash content dropped to7.44%.Millet bran protein isoelectric point is pI=3.5, millet bran albumin molecular weight with15kDa-90kDa, which is relatively higher molecular weight of25.1kDa and15.2kDa subunit content. Millet bran clear protein amino acids, glutamate, followed by essential amino acids leucine, including essential amino acids (in addition to tryptophan) accounted to41.2%of the protein4. The test of anti-chemical liver injury:a healthy Kunming mice were used as the object of study, will be extracted from millet bran albumin mixed feed, fed30d and Created chemical liver injury model fed CCl4to mice,determination of serum alanine aminotransferase (ALT),aspartate aminotransferase (AST) activity and hepatic malondialdehyde (MDA) in liver were observed surface morphology and liver histopathological changes. The results showed that:millet bran albumin has a protective effect on CCl4-induced chemical liver injury and has a dose relationship。5. The test of anti-alcoholic liver injury:a healthy Kunming mice for the study, millet bran albumin and protein hydrolyzate were mixed with the feed, while53°gavage give liquor to the establishment of alcoholic liver injury in mice model, feeding45days, the determination of serum ALT, AST and hepatic MDA, triglycerides (TG) and reduced glutathione (GSH) content, and observe the changes of the liver pathology. The results showed that:millet bran albumin and hydrolysis of bran protein has a protective effect of alcohol due to alcoholic liver injury.