Exploration And Preparation Of The Enhancers Of The Ultra-weak Chemiluminescent Reaction Of Sodium Bisulfite And Hydrogen Peroxide And The Investigation Of Its Analytical Applications

Recently, the investigation of ultra-weak chemiluminescence （CL）between hydrogen peroxide （H₂O₂） and some inorganic salts has attractedmuch attention from a large number of researchers due to their relativelycheap and green reagents and mild reaction conditions. The peroxomonosulfiteintermediate （HSO₄） produced by the reaction of sodium bisulfite （NaHSO₃）and H₂O₂is a highly reactive oxidant, and CL can arise from itsdecomposition. In this work，we explored the enhancement mechanism ofnanomaterials on NaHSO₃-H₂O₂ultra-weak CL system. Moreover, theenhanced CL system was applied to the analysis and detection of real sampleswith satisfactory results. The contents were summarized as follows:Chapter1The main principles of CL, the investigation and application of ultra-weak CL systems, as well as the application of nanomaterials on CLsystems were reviewed.Chapter2Quantum dots （QDs） have become the focus of researchattention due to the unique size-dependent optical and electronic properties，and have been widely used in recent years. In this work, water-soluble CdTeQDs were synthesized in aqueous solution according to the reference withlittle modification, and the enhancement effects of CdTe QDs on theultra-weak CL reaction between NaHSO3and H2O2was explored based on theESR spectra and CL spectra in the absence and presence of CdTe QDs, thechange of fluorescence spectra of CdTe QDs after the CL reaction and theeffects of radical scavengers on CL intensity.Chapter3Compared to fluorescent semiconductor nanocrystals,photoluminescent carbon nanodots are superior in chemical stability,biocompatibility, and low toxicity. In this work, aqueous-dispersible carbonnanodots with high quantum yields were synthesized through solvothermalroute and were applied to NaHSO₃-H₂O₂CL system for the first time. It wasfound that the CL emission intensity can be significantly enhanced by carbonnanodots. We elucidated the CL enhancement mechanism of carbon nanodotson the NaHSO₃-H₂O₂aqueous system based on the fluorescence spectra, CLspectra, ESR spectra and the effects of radical scavengers on CL intensity.Moreover, it was noticed that the addition of rifampicin could inhibit theintensity of carbon nanodots-enhanced NaHSO₃-H₂O₂CL and the quenching rates were dependent on the concentration of rifampicin. Based on thisphenomenon, a simple and rapid flow-injection CL method was proposed forthe determination of rifampicin in eye drops.Chapter4In this work, we established a layer-by layer method tomodify luminescent materials onto plasmonic nanomaterials in order tocombine the stable luminescence of fluorophore with the excellent plasmonicproperty of metal nanomaterials. To illustrate the usefulness of the synthesizednanocomposite, H₂O₂-NaHSO₃CL reaction was chosen as a model system andthe effects of plasmonic luminescent nanocomposite on the CL were exploredfor the first time. The interactions of chemically excited states offluorophores with surface plasmons were supposed to be the possibleenhancement mechanism.