Study On Cell Adhesion With The Substrates On Glass

Because of its important role in biological functions, cell adhesion havebeen applied widely in cytology and tissue engineering. In this work, in orderto improve the adhesion effect of the amine slides, the vapor depositionmethod was developed. Moreover, graphene oxide-chitosan composite filmswas made and covered on the glass hoping to improve cell adhesion effect ofthe substrate. The content of the research work are as follows:(1) In this part, two different deposition methods of3-aminopropyltriethoxysilane (APTES) on glass slides were compared inorder to study the adhesion effect of cervical exfoliated cells on smear slides.Glass slides were modified by vapor-phase deposition (V-D) and liquid-phasedeposition (L-D), respectively. The numbers of cells adhered on the slidesfunctionalized by V-D and L-D were counted and compared under themicroscope. The number of cells on the slides functionalized by V-D and L-Dwas3457±668and2771±693, respectively. The data showed significantdifferences between the two methods (t-test: P <0.05). The results presentedhere have made it theoretically possible to produce amine slides by V-Dmethod for the ThinPrep cytologic test. (2) Graphene oxide were dispersed in chitosan/acetic acid solutions.Graphene oxide-chitosan films were produced. The mechanical properties ofcomposite films were tested by nanoindentation method. With the addition ofa small amount of graphene in chitosan (0.1-2.0wt%), the elastic modulusand hardness of chitosan films were increased. The cell adhesion resultshowed that the HepG2cell and MCF-7cell can adhere to and develop on thegraphene oxide-chitosan composite films as well as on pure chitosan film,indicating that graphene oxide-chitosan composites have goodbiocompatibility. Because there is no metallic impurity in the production stepsof graphene oxide, the time-consuming purification process for removingmetal nanoparticles is thus avoided when graphene oxide is used to preparebiomedical materials. Graphene oxide-chitosan films are potential candidatesas scaffold materials in tissue engineering. As to MCF-7cell, although theGO in GS film rise the adhesion effect, the GO is not good for cell growth andproliferation.