| Angiotensin converting enzyme (ACE) is one of the key kinds of enzyme in resin-angiotensin system and a key target for the treatment of hypertension. Currently, the immobilized enzyme that is a new technology developed in the1950s is mainly concentrated in looking for a good performance of a carrier, a good carrier can significantly improve the enzymatic hydrolysis efficiency of the immobilized enzyme, good carrier of the enzymatic process is more stable.The permeability of monolithic materials is good, which allowe efficient mass transport of solutes within the material. The use of immobilized ACE for screening ACEI has been a hot research area for drug development. Therefore, the aim of this dissertation is to prepare monolithic materials via cryogelation and the immobilization of ACE for ACEI from natural drugs.Our chief research work is as follows:1. A new method was developed to prepare chitosan monolithic columns via cryogelation. The different concentration of chitosan and the amount of glutaraldehyde were optimized to determine their influence on chitosan monolithic columns. The properties of monolithic materials is good,which comform Hangen-poiseuille.2. The macroporous albumin/chitosan monolithic columns were prepared via cryogelation. The monolithic columns were used for Angiotensin converting enzyme immobilization via glutaraldehyde. The optimum conditions of monolithic columns were investigated, the optimum conditions and properties of immobilization enzyme were investigated. The optimum pH, reactiion temperature and temperature stability are better than the solution of ACE.The immobilized ACE can be used repeatedly and stored.3. The macroporous albumin/chitosan monolithic columns which are used for Angiotensin converting enzyme immobilization were prepared via cryogelation. We can fiter ACEI from natural drugs by comparing the differemces of components corresponding to the two peaks associated with Affinity chromatography and HPLC. |
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