Isolation Of Ammonific Ation Bacteria And Its Characterstics Research In Water

Domestic and foreign scholars have done extensive researches on eutrophication, mainly concentrated in the conversion of inorganic nitrogen and phosphorus removal. Lack an effective understanding of the role of organic nitrogen in the water nitrogen cycle. But the more eutrophication water, the more frequently algal bloom, Algae would decompose and release organic nitrogen when it died. How the organic nitrogen quickly converted to ammonia and then through nitrification-denitrification revoming out of the water body? How to quickly remove the water nitrogen has become a relevant research focus.This topic is based on function of ammonification bacteria in the nitrogen cycle, and hopes to enrich and isolate efficient ammonification bacteria which possess rapid transformation organic nitrogen and play a role in any lake of eutrophication water from enrichment of natural water bodies. In this paper, it used the characteristics of ammonification bacteria utilizing organic nitrogen, established the algal cell debris model, choosed ammonification bacteria from eutrophic water by carrying on enrichment, isolation, screening and characteristics of the study. The main results are as follows:1. Used enrichment ammonification bacteria culture medium, enriched and domesticated different environmental samples in one month,100bacteria were isolated by flat-panel, all of which had ammonification capacity. The detection of indicators by testing the generated concentration of ammonia nitrogen, obtained a strain called BH11by using the algal cell debris model, which could efficient degradate the algal debris and had high ammonification ability.2. The physiological, biochemical and16SrDNA sequences of BH11bacteria were analyzed. The results showed that BH11belonged to Delftia acidovorans. It was G", the milky single colonies, the complete edge, round, surface moist, raised. Its genus was the1999new built genus, and there were many features worthy of further study.3. Contrary to the high ammonification ability of BH11do the research on nitrogen transformation characteristics.37.36%algae residues (Microcystis flos-aquae, PCC7806)were removed in the dark condition of28℃,200r/min fostering3days with average ammonification rates0.591mg·L-1·h-1. Heterotrophic nitrification and aerobic denitrification ability were found during further studies, and removing rates of NH4+-N(100mg/L) reaches79.609%with average heterotrophic nitrification rates0.553mg·L-1·h-1in the condition of28℃,200r/min fostering6days. Removing rates of NO3--N (100mg/L) reaches100%with the average aerobic denitrification rates0.694mg·L-1·h-1at the same condition. BH11has the characteristics of ammoniation, heterotrophic nitrification and aerobic denitrification, which has excellent application potential in treating eutrophic water.4. In addition, the study about other characteristics of BH11was carried through. The results showed that BH11had characteristics of degradation aniline. BH11at the condition of28℃,200r/min can degrade aniline of rates45.589%and75.768%at the initial concentration of500mg/L and100mg/L. After incubating20days aniline residues concentration were272.055mg/L and24.232mg/L. BH11has the ability of degradation of the environment poison aniline.