Optimizing Rhamnolipid Production By Pseudomonas Aeruginosa ATCC9027Grown On Waste Frying Oil

Biological surfactant is a kind material，which is producted by microorganisms、plants and is composition of Hydrophilic and hydrophobic.It is usually caused byCultivation of microorganisms under aerobic and anaerobic conditions in differentcarbon source medium,In their metabolic process has some secretion with certainsurface active material.According to the structure characteristics of the biologicalsurface active agent,It can be divided into5categories: glycolipid, lipopeptides andlipoproteins, phospholipids and fatty acids, polymeric surfactant type and particlesurface active agents.Due to the biological surface active agent with solubilization,emulsification and wettability effect, It is playing an increasingly important role inthe petroleum industry, the environmental engineering field, the field of food,medicine, cosmetic fields.In this paper studies,rhamnolipid as a common type of thebiological surface active agent. Which can generally be obtained throughfermentation, and then was extracted and purified by the extraction, ultrafiltration,foam separation, column chromatography.In this study, Rhamnolipid production by Pseudomonas aruginosa ATCC9027with waste frying oil as sole carbon source.The organic synthesis in the waste oil canwere directly use by these microorganisms,and then a special life activity of thebiological surface active agent is producted.Through this experiment,waste oil wastreated, meanwhile the valuable biosurfactant rhamnolipid was gainedIn this study, Rhamnolipid production by Pseudomonas aruginosa ATCC9027with waste frying oil as sole carbon source was studied using response surfacemethod. Cultures were incubated in shaking flask with temperature, NO3-and Mg2+as the variables. Meanwhile, fed-batch fermentation experiments were conducted in2-L Fermentation tank. Results showed that the three variables were closely relatedto rhamnolipid production. The optimal cultivation conditions were of6.4g/LNaNO3,3.1g/L MgSO4at32℃, with the maximum rhamnolipid production of6.6g/L. The results of fed-batch fermentation experiments showed that feeding the oil intwo batches can enhance rhamnolipid production. The best time interval was72hwith maximum rhamnolipid production of8.5g/L. The data were potentially usefulfor mass production of rhamnolipid on oil waste with this bacterium.