Dynamic Light Scattering Of Nanoparticle Used For Ions And Small Molecular Analyses

Dynamic light scattering (DLS) can be used to measure the hydrodynamic sizeand size distribution of nanoparticles and colloids in a liquid environment, includingpolymers, proteins, and nanoparticles, etc. Due to its simple, rapid, washing-freeand high sensitivity as well as results repeatability, this technology, as an analyticaltool is widely applied to the analysis of small ions, proteins and nucleic acids, and soon. Fluorine is one of essential trace elements for human, but excess fluoride cancause fluorosis to human health, therefore development a fluorine ion detectionmethod is important. At the same time, glucose is the main energy substances inorganism, but also is the important biological information molecules, which areinvolved in many physiological processes. So glucose plays a great role in humanhealth. This paper intends to detect fluoride ion and glucose based on dynamic lightscattering technology. The main content is shown below.(1) Sensitive fluoride ions detection based on dynamic light scattering ofquantum dotsThe cysteamine-capped CdTe quantum dots aggregates can be disaggregated inthe presence of fluoride ions, which lead to the change of hydrodynamic size. Basedon this, a sensitive fluoride ions detection based on dynamic light scattering ofquantum dots was established. The method was washing-free, sensitive, specific, andthe detection limit of20.0nmol/L for fluoride can be obtained. Compared withelectrode method, the detection limit of this approach was down to about2orders.This work expanded the detection objects of the DLS technique.(2) Sensitive glucose detection based on dynamic light scattering of boronic acidfunctionalized gold nanoparticlesAt first, water soluble and high stable gold nanoparticles capped with boronicacid were prepared. Due to the specific binding of boronic acid with glucose, presenceof glucose can lead to the aggregation of boronic acid functionalized goldnanoparticle, which resulted in the signal enhancement of light scattering. Based onthis, a novel glucose detection strategy was developed. This method is fast andsensitive homogeneous assay, and the detection limit of6.50nmol/L can be reached.Compared with fluorescent and colorimetric method, the detection limit of thisapproach was down to about3orders The method can also be used for glucose sensingin serum.