Reconstruction For Genome-scale Metabolic Network Of Gluconobacter Oxydans

Genome-scale reconstruction and analysis of a metabolic netawork is greatsignificance for the study of systematic metabolic or guiding for the metabolicengineering for strain improvement.Gluconobacter oxydans is widely applied in biotechnology industry, because ofits ability to incompletely oxidize a great variety of carbohydrates, alcohols andrelated compounds. Gluvonobacter oxydans can be used in the production of Varietyof products, such as1,3-dihydroxyacetone, vitamin C, Miglitol and so on. However,the systematical cellular metabolism in G.oxydans remains unclear, hindering itswidely practical application. To further our understanding of such physiologicalcharacteristics, a genome-scale metabolic network (named iXW433) of G. oxydans621H based on the genome annotations and biochemical information wasreconstructed and validated in this paper.The reconstructed model iXW433includes433genes,859reactions and985metabolites. The intracellular reactions classified into73specific pathways whichbelonging to five major metabolism subsystems. The predicted biomass growth ratesin close agreement with the experimental values when the concentration of glycerol ata low level, shows that the model have high confidence.The topological structure graph was drawn using Pajek program, the structuregraph contain896nodes and1223lines. The connection degree distribution of theiXW433was considered to be agree with power-law distribution, so iXW433belongeto scale-free network.8metabolites which have higest degree of connection werethought bo be the potential hug metabolites. The relation between glycerol,NH3, DHA,biomass and ATP in the metabolic network was studied by using Robustness Analysis,and the simulated result of growth rate, DHAsynthesize rate andATP synthesize ratewas found agree with the phenotype.105reactions were found to be flux stablereactions while194other reactions were found to be flux variability reactions.92essential genes,137essential reactions and55double-genes lethal pairs were foundin IXW433.