Preparation And Application Research Of Polymer Micro/Nano Fibers By Laser Melt Electrospinning

Laser melt electrospinning method is a kind of environmental protection, high efficiencyand more ideal method to prepare micro/nano fibers. This method is a solvent free meltelectrospinning process, realizes the fiber diameter can be modulated, fiber non-toxic and highproduction efficiency. Compared with the solution electrospinning, it is more efficient,environmental protection and safety. Biodegradable micro/nano fibrous scaffold materialsprepared by Laser melt electrospinning are expected better application in tissue engineering.In present paper, we used laser melt electrospinning technology to prepare Nylon6(PA6),Thermoplastic polyurethane (TPU), poly ethylene glycol-poly lactic acid copolymers(MPEG-PLLA) and poly lactide-co-glycoli cacid (PLGA) fibers. The effects of electrospunparameters on the fiber diameters were investigated. The physi-chemical properties of the fiberswere also studied. In addition, we also use nanometer hydroxyapatite (nHA) to modify PLGAfibers, and obtained the modified PLGA/nHA composite fibers, Hope to improve the mechanicalproperties and hydrophilicity of electrospun fibre membranes. At the same time, we haveproformed the cell culture experiment test on the PLGA/nHA composite fiber materials andstudyed its biocompatibility.We use Scanning electron microscopy (SEM) to observe the morphology of fibers, and useFourier transform infrared (FTIR), differential scanning calorimetry (DSC),X-ray diffraction(XRD) and energy spectrum analysis (EDS) to character physi-chemical properties of the fibers.Tensile behaviors are also studied. The results showed that: for PA6fibers, the fibers diametertended to decrease as laser current increased. For TPU and MPEG-PLLA fibers, applied voltagewas not statistically significant effect on the TPU and MPEG-PLLA fibers diameter. XRD andDSC results showed that the crystallinity of melt electrospun PA6mats was around48.7%.However, TPU and PLGA fibers exhibited an amorphous phase, respectively. The EDS analysis confirmed the presence of nanoparticles in the PLLA/nHA fibers. The mechanical test showedthat, for TPU and PLGA mats, the breaking strain were133.85±36.2%and14.71±6.74%,respectively. the average tensile strength were3±1.24MPa and1.02±0.35MPa, respectively.The results of orthogonal design showed the order of significance levels on PLGA fibers asfollows: laser current, feed speed, applied voltage. Porosity calculation and degradationexperiments showed: PLGA electrospun fiber membrane scaffolds have high porosity and goodbiodegradable properties; Water contact angle experiments showed that adding HA can improvethe PLGA electrospun membrane hydrophilic at some extent. Cell culture experiments showedthat: the laser melt electrospinning of PLGA/nHA micro/nano could promote the growth andreproduction of human embryonic kidney cells. Fibrous scaffold materials could provide idealcell adhesion, proliferation and differentiation environment for cells.