Research On The Separation Of Ionic Compounds Using Porous Graphite Carbon Column

As a new kind of stationary phase, porous graphited carbon has the unique physical and chemical characteristics. Aimed at probing into the separation conditions of different types of ionic compounds with porous graphited carbon column, the chromatographic analysis methods of ionic compounds such as anions, organic acids have been established. By the means of exploring its separation mechanism preliminarily, the ionic compounds analysis methods establishment has been further guided. So that the separation conditions fit for different kinds of analytes can be established quickly and precisely.Firstly, this article discusses the retention behavior of inorganic anions with porous graphited carbon column. The separation mechanism of anions has been confirned by comparing different chromatographic conditions. The retention behaviors indicated that both electrostatic interaction and hydrophobic interaction were acted as major function factors involved in the separation of inorganic anion. The chromatography method with suppressed conductivity detection and UV detection to separate anions was developed. By discussing the retention behavior of organic acids, the mobile phase selection method and the good aromatic acid isomers selectivity of PGC column were confirmed. The method to separate7organic acids has been developed with phosphate as mobile phase and ultraviolet detection. Disodium hydrogen phosphate and methanol were used as mobile phase, aromatic acids and their isomers have been separated successfully.Finally, the conclusion and method discussed above were applied in the amino acid and sugar material separation, and the conclusion was further verified. The application scope was expanded to the detennination of biological sample, which is of great practical value.