| Based on the porous polystyrene microspheres, submicron polystyrene magneticmicrospheres have been synthesized by using Fe3O4nanoparticles with differentsurface modifications as magnetic material. Further magnetic separation experimentsby using the immune magnetic macrospheres coated with salmonellaspecificantibodies showed highly separation efficiency on salmonella samples.Firstly, by using Fe（acac）3（iron acetylacetonate） as metal organic precursor, PEG,PVP and PVP/triethylene glycol coated iron oxide nanoparticles have beensynthesized through the thermal decomposition method respectively. The magneticproperty of the above three magnetic nanoparticles was analyzed. The results showedthat the PVP/triethylene glycol coated iron oxide nanoparticles were characterized bythe highest saturation magnetization intensity, which is more suitable to be used forpreparation of magnetic microspheres than the others.Secondly, monodisperse magnetic polystyrene microspheres have been successfullysynthesized by adding the iron oxide nanoparticles into the dispersionpolymerization reaction systemof preparing porous polystyrene microspheres. PVPand PVP/triethylene glycolcoated iron oxide nanoparticleswere respectively used forthe preparation. The magnetic property and colloid stability of acquired magneticpolystyrene microspheres were analyzed. The results showed thatmagneticpolystyrene microspheres by using PVP/triethylene glycolcoated iron oxidenanoparticles as materials were characterized by better magnetic response, which ismore suitable for the preparation of immunomagnetic microspheres.Finally, through the electrostatic interaction between magnetic beadsandanti-Salmonella CSA-1antibody， submicro immunomagnetic beads werepreparedand applied in separating Salmonella sp. |
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