Research On The Construction And Applications Of Naphthalimide-Rhodamine Energy Transfer Cassettes

In recent years, fluorescence analysis method to detect metal ions and biological molecules have been more and more concerned. Fluorescence detection technology has been widely used in biological imaging, biological monitoring, immune analysis and environmental protection and so on. Although turn-on probes are more sensitive due to the lack of background signal, a major limitation is that variations in the sample environment (pH, polarity, temperature, and so forth) might influence the fluorescence intensity measurements, the exploration of multifluorophores with energy donor-acceptor architectures can achieve large pseudo-Stokes shifts, meanwhile affording simultaneous recorded ratio signals of two emission intensities at different wavelengths, which could provide a built-in correction for the environmental effects. Forster Resonance Energy Transfer (FRET) is generally the most adopted methodology for addressing this issue.Based on a through bond energy transfer (TBET) and a through space energy transfer between rhodamine and a naphthalimide fluorophore, two fluorescent ratiometric chemodosimeters RNl and RN2were designed and prepared for single selective detection of Cu2+and Fe3+in aqueous solution and in living cells, respectively. They did not interfere with the fluorescence ratio by other ions. The fluorescence intensity of rhodamine increased after added correspond metal ions into aqueous solution of fluorescent probes. In addition, Upon addition of EDTA into the aqueous solution of RN2that had already added Fe3+, the fluorescence intensity of rhodamine decreased that demonstrated the probe RN2could enable reversibly detection for Fe3+.