Study On The Fluorescencence Sensor For Ruthenium

Ruthenium is one of the platinum group metals and is widely used in electronics industry, chlor-alkali industry, automotive industry and other fields, meanwhile, ruthenium catalyst especially Grubbs-catalyst, as an important class of catalyst, is widely used in chemical industry, environment, medicine and other fields. The detection and identification of ruthenium are directly related to the assessment of residue levels of ruthenium and ruthenium pollution control, ruthenium quantitative detection has attracted researchers more and more attention.Currently, the detection methods of the ruthenium including spectrophotometry, electrochemical method, mass spectrometry and atomic absorption spectrometry. These methods usually require large-scale analytical instrumentation, complex sample preparation process, and specially trained personnel to operate so that they are difficult to be universal applicated. In recent years, the fluorescent probe, as a simple, sensitive and efficient means of detection, has been studied more and attracts more and more attention.In this paper, we used olefin oxidation reaction of ruthenium to design and synthesize ruthenium fluorescent probes C1and C2by connecting an olefin group to fluorescein derivatives. In a phosphate buffer, C1and C2could detect ruthenium with a high selectivity and a good immunity in the prescence of other anions and cations and the response time to ruthenium was1hour. The detection limits of C1and C2were3.9×10-8mol/L (39ppb) and8.7×10-8mol/L (87ppb) respectively. Therefore, C1and C2were basically successful as ruthenium fluorescent probes. Unsatisfied as we are, we try to optimize the two compounds previously metioned. After shortening the carbon chain of the olefin group, the other ruthenium fluorescent probe C3is synthesized. C3had a bigger change in the fluorescence intensity as expected and also it had a good selectivity and immunity to other anions and cations. The response time downed to30min and the detection limit downed to3.2×10-10mol/L (0.32ppb) compared with C1and C2, in other words, it was a better ruthenium fluorescent probe. We confirmed that C3can detect residue Grubbs-catalyst2nd in product which was synthesized from olefin methetasis reaction. Finally, we compared the detection efficiency on resuide ruthenium (Grubbs-catalyst2nd) of C3with that of ICP-MS, the results prescent that our fluorescence method had’a higher sensitivity.