A Capillary Array Electrophoresis LIF Detection System With Automated Sample Introduction

Capillary electrophoresis (CE) has been widely used in many fields of analytical chemistry due to its high separation performance. In recent years, the emergency of the capillary array electrophoresis (CAE) has increased the analysis throughput by scores or hundreds of times, which directly contributed to the accomplishment of the Human Genome Project (HGP) ahead of schedule. In this paper, an automated, fast, high-throughput and simple capillary array electrophoresis system was developed with a two-dimensional slotted-vial array sample introduction, the optical system of which was simplified by using a laser generating line pattern beam and the liquid-core waveguide (LCW) technique.In Chapter1, the development of capillary electrophoresis, chip-based capillary electrophoresis and CAE was reviewed. The scanning and imaging laser induced fluorescence (LIF) detection methods of CAE were fully discussed. The sampling techniques of CAE, the fixed sample reservoir method and the sampling probe method, were summarized.In Chapter2, a capillary array electrophoresis system with automated sample introduction and LIF detection method was established. An eight-capillary array electrophoresis chip was designed and fabricated. The fluorescence was transmitted to the end of the LCW capillaries by LCW technique and detected subsequently. A two-dimensional slotted-vial array sample introduction device controlled by computer was designed. The inlets of capillaries were used for sampling, and the continuous electro kinetic sample injection was achieved. A line-patterned laser emitted by a diode laser was used to irradiate all the capillaries simultaneously requiring no movable parts. An8-channel linear array multianode Photomultiplier Tube (PMT) with high sensitivity was introduced to detect the samples in all the capillaries at one time. The CAE system was used to separate the mixture of Rhodamine6G and Rhodamine B with the limit of detection3.3x10-11mol/L and the migration time RSD of Rhodamine6G in different channels8.1%(n=8). The DNA marker of DL2000and the PCR products were also well separated using this system. DNA Marker of DL2000can be analyzed in7minutes,and the throughput was64/h with a carryover of2.8%.Only13.6nL sample solution was introduced for each assay when1-s sampling time was adopted.In Chapter3,the capillary array electrophoresis LIF detection system with automated sample introduction for was summarized and its prospective application was discussed.