| Detecting protein or other macromolecules with high sensitiv-ity and specificityis essential for disease diagnostics, drug screening, and other applications.Surface-enhanced Raman scattering （SERS） has been widely used in the ultrasensitivedetection of various chemical and biological molecules, because it provides abundantchemical and structural information of molecules. This technology has obviousadvantages such as narrow spectrum, high sensitivity and selectivity, low injure. Inorder to realize application of detection of protein molecules, the most critical aspectis fabrication of SERS-active substrates with large SERS enhancement, highsensitivity, fine uniformity, good reproducibility, and biocompatibility. Generally,SERS intensity is related to size and shape of metal nanostructures and distancebetween analyte molecules and metal surfaces. Even though traditional SERSsubstrates, such as roughened metallic surfaces, metal islands, fractal films, canprovide SERS enhancement, the highly ordered SERS substrates with reproduciblewith much higher Raman enhancement are needed for measurement of trace-levelanalytes. Au nanoshell materials consist of a dielectric core covered a thin Au shelland have been successfully employed in a range of biomedical application. Theplasmon resonance wavelength of Au nanoshells can be varied by controlling therelative inner and outer radius of their metallic layer in the near-infrared window. It isimportant for bioapplication because the blood and tissue are maximally transparentand light can penetrate tissue to depths of several inches. Besides their tunable opticalproperties, a great enhancement of Raman scattering cross-section of adsorbedmolecules can be achieved by applying Au nanoshell as SERS substrates.In the paper we had prepared different thickness of SiO2/Au nanomaterialsthrough gold nanoparticles absorbed on the surface of SiO2with APTMS. Thesematerials were characterized by the UV-vis absorption,SEM,TEM,Mapping andEDX.We had presented the appliation of SERS detection for analysis ofaminothiophenol based on gold nanoparticles and SiO2/Au nanoshell, and the SERSdetection was studied by changing the concentrations of aminothiophenol based onSiO2/Au nanoshells.Using fluorescein isothiocyanate （FITC） Raman signal as featureto recognise signal for protein detection sensitivity by assembling SiO2/Au proteinprobes.Then we analyzed the antigen recognition of the different antibodies throughthe experiment. |
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