| High pressure processing (HHP) can complete the extraction process at low temperature, which means HHP can keep the activity of extract. So, we applied the HHP into extract astaxanthin from shell, to study the influence of factors (pressure/pressure holding time/solvent/sample diameter/liquid to solid ratio) on the yield of astaxanthin; Then detect the component and antioxidant change of astaxanthin solution affected by high pressure processing; at the last, detected the microstructure of shell to discuss the mechanism. The results are as follows:1. The ability of solvent is ethanol>acetone>dichloromethane. Pressure affected the extract ability of ethanol obviously. When the sample diameter was smaller than0.45mm, the yield of astaxanthin was very high. Liquid to solid ratio has negative influence on the yield of astaxanthin when the ratio was high.2. Built a model by quadratic orthogonal rotation combination design.Pressure, pressure holding time and liquid to solid ratio were used to build the model. Liquid to solid ratio in secondary was significant (P<0.05), and the interaction between each two factors also had obvious influence on the yield of astaxanthin. The optimum condition is,210MPa,32mL/g,9.2min in ethanol, the forecast and actual yield of astaxanthin were89.46ug/g and89.12μg/g, the relative error is1.08%. The model was predictability and repeatability.3. The astaxanthin solvent was detected the components and antioxidant. The results showed that the solvent by high pressure is simpler than others. And the antioxidant of astaxanthin in vitro measured by remove DPPH radical and superoxide anion radical, the results showed that, the antioxidant of astaxanthin treated by high pressure was protected and higher than treated by ultrasonic and control.4. The microstructure of shrimp shell was observed to find the influence of high pressure processing. The results showed that micropores were expanded at lower pressure, thereby expanding the dissolution channel of astaxanthin. |
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