Purification And Decoloirzation Of Sasanquasaponin

Sasanquasaponin extracted from the Camellia oleifera seed pomace is a naturalsurfactant with excellent performances of effective in decontamination, foaming,emulsification, decentralization and saturation. It also has some biological activities suchas anticancer activity and antiallergenic, antibacterial, anti-inflammatory andantihepatotoxic activities. It is a potential resource with huge economic value. The pomaceis in addition to sasanquasaponin rich, but also rich in flavonoids, tannins, protein,polysaccharide and some other impurities. So it is hard to purify the sasanquasaponin andlimit the scope of its application because of the color of sample and its purity. It has beenwidely used in aquaculture, building materials, daily-use chemical and other aspects. Butthe value of medicinal and food have not been widely exploited. More attention should bepaied on the purification and decolorization effectively to make full use o f its potentialvalue, and bring us social and economic benefits.AB-8macroporous resin was used in this experiment for standard sasanquasaponinpurification, for there is no standard sasanquasaponin on market right now. High-puritysasanquasaponin was obtained by methanol-acetone recrystallization after macroporouspurification. The sample was detected by thin-layer chromatography (TLC), UV-Visscanning, LC-MS and some other method. There was only one point on TLC under sixexpend agents; an absorption peak in210nm on the UV-Vis scanning Chromatogram justwas in line with the literature reports; color reaction showed that the sample wastriterpenoid compounds; the compound with15.99min retention time was1202m/z;weight detection was99.3%. All the results showed that the sample was sasanquasaponinwith high purity. Standard curve was determined by vanillin-sulfuric acid colorimetricmethod. The linear relationship of the standard curve was well, and the standard curvecould be used for purity detection during the experiment.Ceramic membrane was used for purification crude sasanquasaponin. Saponin transferrate, impurity removal rate and purity were three indexes evaluated by pore diameter,transmembrane pressure and concentration. The optimal conditions were0.05μm ceramicmembrane,1.0MPa transmembrane pressure and1.0%concentration. Then the filtrate wasconcentrated and spray-dried. The purity of sasanquasaponin has been great improved fromabout50%to83.3%and the yield was69.8%. The sample color of purified bymembrane was better than crude sasanquasaponin. The optimal membrane purification condition was suitable for industrial production.Laccase decolorization and reduction decolorization were evaluated to decolor thepurified sample. And the results of two special methods were compared with H2O2decolorization, which was the best for now. The result showed that laccase decolorizationwas not suitable for sasanquasaponin. The color of sasanquasaponin got worse with laccaseloading and like red wine. While sasanquasaponin could be decolored effectively byreducing agent. Response surface methodology (RSM) was used to optimize the bestdecolorization condition by SAS9.1.3software base on single factor experiments. Theoptimal conditions were1.0%KBH4loading,2.0%NaHSO3loading, pH at6.0andreaction temperature at40.0℃. The result of this study was L*value was80.0and thedecolorization rate was51.8%. Validation experiments’ results agreed with the modelprediction. Comparing with the sample decolored by H2O2, reduction decolorization hassome advantages such as effective, fewer agents consuming and little affect tosasanquasaponin which does not decrease its tensiometric property. The reductiondecolorization performance well and could be used in industry.