Preparation Of N-succinyl-chitosan Nanoparticles And Research On The Activity And Anti-tumor In Vitro

Chitosan is a natural polymer and it derives from deacetylated chitin. Chitosan was applied in the study of drug carrier in recent years because of its low toxicity and good biocompatibility. However the applications of chitosans has been limited in fields of medicine and others because of the spatial structure of no rules and the huge molecular mass resulting in its poor solubility. N-succinyl-chitosan is a N-acylating derivative of chitosan and synthesis derived from chitosan and succinic anhydride. Compared to chitosan N-succinyl-chitosan has greatly improved solubility besides the vivo compatibility, non-toxic and strong adhesion as same to chitosan thus it broadens the range of applications of chitosan. So it is an excellent body medium which has open prospects for drug-loaded.Firstly, purchased raw chitosans were removed of acetyl with a batch of concentrated soda prepared by a high deacetylation degree of chitosan, measured by a simple acid-base titration obtaining the degree of deacetylation increased from88.65%to97.62%, and its infrared characterization has been basically acetyl removed from the IR spectra analysis; Then the relative molecular mass of the high deacetylation degree of of chitosan was degragated by hydrogen peroxide oxidative degradation obtaining low molecular weight chitosan by different degradation time. The high degree deacetylation of chitosans and low relative molecular weight of chitosans were mearured by molecular weight with Ubbelohde viscosity concluded that chitosan-derived molecular weight degradation with the extension of time and greatly reduce the conclusion, after that infrared characterization and analysis from the IR spectra of hydrogen peroxide the basic degradation method to maintain the structural integrity of chitosan molecules. Experiment was carried out by the synthesis of N-succinyl-chitosan and characterization with the selection of degradation20min chitosan and succinic anhydride as raw materials,which molecular weight is21,000. Synthesis of N-succinyl-chitosan was in the acetic acid/acetone system at room temperatureobtained by the potentiometric titration measurement of its isoelectric point range of3to8.2, obtained from IR spectra analysis via infrared characterization of chitosan has already been successfully introduced at the N-bit succinyl prepared enlightenment N-succinimidylacyl chitosan, NMR Characterization, obtained from the1H-NMR spectrum analysis of the N-succinyl-chitosan degree of substitution of0.58, N-succinyl-chitosan thermal decomposition temperature is256℃by thermogravimetric analysis.N-succinyl-chitosan nano was induced by ion Preparation of N-succinyl-chitosan nanoparticles with the morphology of the drug loading and drug carrier. Experimentally obtained N-succinyl-chitosan nanoparticles was measured by the particle diameter reaches149.4nm, the surface potential measured by the potential analyzer9.99mv, nanoparticles system is more stable, TEM analysis of the nanoparticle surface morphology can be seen from the lens photo cashiermorphological integrity of a grain of rice; also prepared a load of recombinant human endostatin N-succinyl-chitosan nanoparticles derived from the TEM images of drug-loaded nanoparticles and blank nanoparticles form, the good performance of the drug, the other, endostatin protein quantitative assay of N-succinyl-chitosan nanoparticles encapsulation efficiency the average nanoparticle encapsulation efficiency was94.76%.N-succinyl chitosan nanoparticles in vitro cell activity and toxicity evaluation of N-succinyl-chitosan nanoparticles and carrier of recombinant human endostatin vitro anti-tumor activity of MCF-7tumor cells. In addition, by MTT assay N-succinyl chitosan nanoparticles blank drug carriers NIH normal cell proliferation, the experimental results show that different concentrations of nanoparticles suspension cell reproductive role, but the inhibition rate are small, prove that N-succinyl chitosan nanoparticles on normal The cells had no toxicity. Last inspection by MTT assay in vitro anti-tumor activity of N-succinyl nanoparticles upload recombinant human endostatin, endostatin is a potent exogenous tumor angiogenesis inhibitor, experimental results show that the drug-loaded carrier inhibition of tumor cells MCF-7reproductive role better than the same concentration of endostatin dope, N-succinyl-chitosan nanoparticles as drug-loaded carrier, endostatin inhibition of tumor cells has been strengthened, the results further demonstrate the feasibility of the N-succinyl-chitosan nanoparticles drug loading body.