Determination Of Phenols Of Environmental Hormones In Industrial Wastewater By SPME-HPLC

In the past several years, because of the development of high-speed of chemicalsynthesis technology, most animals and humans are under the threat of environmentalhormone “ocean”. Animals and human beings have been severely affected by theenvironmental hormone pollution, sexual function of organisms decreased, sexualorgans alienation phenomenon have occurred frequently. For the past20years, theserious effects of environmental hormones on human gradually emerged, neither aman nor a woman sexual dysfunction and disease incidence rises on a global scale,which has aroused the concern of environmental hormones. Studies on environmentalhormones at present mainly focused on environmental investigation，toxicologicalstudy and establishment of estimate method. However, controlling and removal ofenvironmental hormones in environment is short of study accumulation. The thesisaimed at phenolics of treating environmental hormones in industrial wastewater.Nonylphenol,2,4-dichlorophenol,2,4,6–trichlorophenol were selected as the modelpollutants which have environmental hormones effects and exist generally inindustrial wastewater. A sample and highly method was established for thedetermination of nonylphenol,2,4-dichlorophenol,2,4,6–trichlorophenol inindustrial wastewater. This study provided valuable information to the practicalremoval nonylphenol,2,4-dichlorophenol,2,4,6–trichlorophenol in industrialwastewater by these three processes.A simple and highly sensitive method has been developed for the determinationof nonylphenol,2,4-dichlorophenol,2,4,6–trichlorophenol by high performanceliquid chromatography （HPLC） with UV. Nonylphenol detection in the wavelength of222nm for UV detection. A standard curve was=185.78415X+138.89329，R=0.9992;Concentration of nonylpheno was0.0944μg/mL in industrial wastewater. Addingstandard solution, the recovery rate of nonylphenol in industrial wastewater was91.90～104.30%, the relative standard deviation range of detection is0.89%.Thedetection limit of nonylphenol in samples is0.0267μg/L.2,4-dichlorophenoldetection in the wavelength of284nm for UV detection. A standard curve was Y= 45.44X+172.91， R=0.9994; Concentration of2,4-dichlorophenol was0.3228μg/mL in industrial wastewater. Adding standard solution, the recovery rate of2,4-dichlorophenol in industrial wastewater was94.36～100.52%, the relativestandard deviation range of detection is1.34%.The detection limit of2,4-dichlorophenol in samples is0.0404μg/L.2,4,6–trichlorophenoldetection in thewavelength of294nm for UV detection. A standard curve was Y=40.72583X+265.55818，R=0.9993; Concentration of2,4,6–trichlorophenol was0.2430μg/mLin industrial wastewater. Adding standard solution, the recovery rate of2,4,6–trichlorophenol in industrial wastewater was90.14～108.3%, the relativestandard deviation range of detection is1.29%.The detection limit of2,4,6–trichlorophenol in samples is0.0388μg/L.Different methods were researched about degradation of nonylphenol,2,4-dichlorophenol,2,4,6–trichlorophenol in industrial wastewater. nonylphenol: studyon removal of nonylphenol using ozone-activated carbon process, by comparingdifferent dosage of ozone, the ozone oxidation time, the type of activated carbon anddosage of activated carbon final optimal conditions,then the final removal ratereached94.81%.2,4-dichlorophenol:degradation of2,4-dichlorophenol in wastewaterby Fenton Reagent-UV, by comparing UV light exposure time, the concentration ofH₂O₂and FeSO4.10H2O in the Fenton’s reagent, ph of the solution final optimalconditions, then the final removal rate reached94.78%,2,4,6–trichlorophenol: studyon removal of2,4,6–trichlorophenol by modified activated carbon, by comparing themodified activated carbon type, dosage of activated carbon, pH of the solution finaloptimal conditions,then the final removal rate reached83.97%.