Preparation And Application Of Ofloxacin Molecularly Imprinted Polymer

Recently, people pay more attention on the residue of antibiotics. Since it will lead small amounts of drugs into the environment inevitably, and ultimately poses a significant threat to human health through the food chain, tracing amounts of drug residue analysis become more prominent issue.However, for precise quantification of some complex biological samples which need the processes of separation and enrichment, MIP material is good choice which could solve these promblems.In this thesis, in order to improve the enrichment and separation efficiency of trace components in complex biological samples, two molecularly imprinted polymers (ofloxacin as template molecule) are synthesized and coupled with high performance liquid chromatography (HPLC) to determine the fluoroquinolone drugs in milk sample. The more detailed novelty of these researches can be categorized as following:1. A novel complex molecularly imprinted polymer (MIP) was prepared using ofloxacin as template molecule, methacrylic acid and2-vinylpyridine as combined monomers and ethylene glycol dimethacrylate as cross-linking agent. The imprinted polymer was characterized by TEM. Compared with the TEM characterization and the static adsorption assay of single functional monomer MIP, the MIP with combined monomers has a sphere shape and a better adsorption capacity and selectivity. The MIP was applied to be concentrating fluoroquinolone in milk sample. A corresponding HPLC analytical method to determine fluoroquinolone has been developed too.2. Using silica nanoparticles as matrix, ofloxacin as template molecule, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linking agent and Dibutyl Terephthalate as plasticizer agent, a novel hollow molecularly imprinted polymer was prepared by removing the silica matrix. The imprinted polymer was characterized by TEM and static adsorption capacity. We found the hollow MIP has uniform sphere shape, good flexibility and dispersion. Furthermore, the hollow MIP has a very high adsorption capacity and mass transfer property to fluoroquinolone. We chose the hollow MIP as an adsorbent to concentrate fluoroquinolone in sample from milk and obtained a good result.