A Study On The Effect Of PFOS To Enzyme Activity And DNA Methylation In Glyptocidaris Crenularis Vivo

In coastal marine environments, persistent organic pollutants (POPs) sedimentation and accumulation characteristics, leading tooffshore sediment pollution is increasingly serious, POPs pollution has become a threat to global marine environment.which is one of the most serious pollution problem. To explore perfluorooctane sulfonate (PFOS), one of the new persistent organic pollutants may cause pollution to the marine biological damage mechanism, and to find biomarkers for detection of PFOS pollution.This article selects patterns of marine benthic environmental biological Glyptocidaris crenularis as subjects, respectively from the organizational level and molecular level research systematically the different doses (0.01,0.1and1mg/L) PFOS pollution to Glyptocidaris crenularis apparent change, the immune system and the impact of gonad genome-wide DNA methylation level, the influence of the main research contents and results are as follows:1. From the organizational level, to explore the effects of PFOS pollution caused to Glyptocidaris crenularis apparent change and immune system, searching the appropriate PFOS pollution biomarkers. employ the method of low dose long exposure, select superoxide dismutase (SOD), catalase (CAT), alkaline phosphate (AKP) and acidic phosphate (ACP) enzyme activity as test indexes, the results show that:Along with the extension of exposure time, the experimental group Glyptocidaris crenularis out sting and the fluid inside the cell morphological changes more and more serious, red blood cell quantity reduce, movement and feeding capacity weakened gradually, the recovery in recovery period.Different doses of PFOS effect on SOD enzyme activity showed a trend of gradual decline, reached the lowest in21d, recovery recovered; ACP activity showed a fall after rise of first; CAT enzyme activity change regularity is not obvious, generally showed lower before rising trend; AKP activity showed a trend of rising first decreases after basically. From the above conclusion. Glyptocidaris crenularis fluid changes of SOD and ACP activity is relatively sensitive, suitable for monitoring Marine pollution of PFOS biomarkers.2. From the molecular level, this article selects gonads as the research object, which is more sensitive to pollutants, employs the Methylation Sensitive Amplified Polymorphism (MSAP) technology, after exposure to PFOS, Glyptocidaris crenularis genome-wide CCGG sites methylation levels are detected. The results show that after exposing to the low, medium and high concentration of PFOS pollutants, along with the extension of exposure time, genome-wide DNA methylation polymorphic rate, CG site methylation rate and demethylation rate, CNG site methylation rate and demethylation rate, compared with the control group showed a trend of increasing, reached the maximum on day21, convalescence began to recover on day28. Compared to control group, low, medium and high concentration group in the methylation rate/to demethylation rate ratio showed a trend of gradual decline in the first21days, reached the lowest on day21,120.59%,128.57%and155.56%respectively. The above results show that a low dose of PFOS exposed for a long time can make Glyptocidaris crenularis DNA methylation level was increased, which could lead to follow-up the genetic toxicity effect.