| Antibiotics play a huge role in the treatment of infectious diseases in human and animal.With the extensive use and abuse of antibiotics, the content of it increased in differentenvironments such as water, soil, vegetation, from different kinds of effluents, which leads to thechanges in microbial community structure, the occurrence of multi-resistant bacteria and thedestruction of the balance in ecosystem. Water environment is the media of resistant bacteria andresistance genes. In recent years, water environment seriously contaminated by antibiotic, and theantibiotic resistant bacteria will carry the resistance gene which is transmitted through the transferelement to pathogens, to expand the scope of drug resistance and enhance the resistant effect,resulting in increasing difficulty of treatment in bacterial infectious diseases, especially in urbanwastewater where the presence of resistant bacteria pose a serious hazard to human health.Enterobacteriaceae as the dominant bacteria in the urban sewage, were mostly pathogens, whichplay a leading role in the spread of drug resistance. Previous reports showed that antibioticresistance to some antibiotics, or the detection of a type of resistance genes in Enterobacteriaceaeisolated from the urban sewage. However, there are few studies about the antibiotic resistance ofvarious antibiotic and the detection of different kinds of drug resistance genes.The aim of study was to disscuss the in Enterobacteriaceae resistance and distribution ofresistance gene urban sewage, Enterobacteriaceae were isolated and identified from15sample sitesof urban sewage in a city, with the values of minimum inhibitory concentration (MIC) on18kindsof antibiotic (Ampicillin, piperacillin, ceftazidime, cefotaxime, cefepime, cefoxitin, ertapenem,meropenem, imipenem, amikacin, gentamicin, tobramycin, ring ciprofloxacin, nitrofurantoin,aztreonam, tetracycline, doxycycline for guitar and multi myxomycetes) and the10resistance genesof tetracyclines, macrolides and beta-lactam of Enterobacteriaceae isolated from15sample sits inurban sewage, which were selected and identified by Gram staining and biochemical experiments,in order to effectively control generation and transmission of resistant Enterobacteriaceae. Theresults showed that:1:667isolates of Enterobacteriaceae were selected and identified in the urban sewage, including24kinds of species (Escherichia coli, Klebsiella pneumoniae, Citrobacter,Enterobacter cloacae, Proteus mirabilis, Klebsiella oxytoca, shaped Citrobacter, Serratia flavor,liquefied Serratia bacteria, Enterobacter aerogenes, Proteus vulgaris, Clifford Harrison leavesMickelson coli, Klebsiella smelly nose, Yersinia bacteria, Guam terbutaline Anglesey coli,malonyl-negative citrate Morgan Morgan bacteria, crimson marcescens, Serratia marcescens,Yersinia intermedia, aquatic Rann coli, Edwardsiella tarda, Plymouth Mao Si Shalei coli andintestinal cell Gao Fei bacilli), and the Escherichia coli and Klebsiella pneumoniae was thedominant bacteria.2: The results of MIC showed that all the Enterobacteriaceae isolates had different resistantlevels to18kinds of antibiotic and there were663multi-drug resistant isolates, contented29antibiotic resistance patterns, which were owned by dominant bacteria mostly.3: The calculation of multi-drug resistance index showed that the value of it in differentsamples was different. The value of S11was highest, which showed that Enterobacteriaceaeresistance and resistance genes are closely related and the promotion of pharmaceuticalwastewater constitutes an important factor in the spread of resistance genes.4: The decection of resistance genes showed that the highest and lowest frequency ofpositive isolates were detected of tetA gene and blaNDM-1gene, respectively, and highestfrequencies of all the genes in S11, which investigated drug resistance of Enterobacteriaceae isclosely related to the occurrence of resistance genes, and there was potential risk inpharmaceutical wastewater.5: Two blaNDM-positive bacteria, including Escherichia coli KR5.12and Klebsiellapneumoniae NF241942were detected in this study, and the fitness of the two strains reduced withthe increasing times of generation.In summary, Enterobacteriaceae isolated were resistant to various antibiotic, and multi-drugresistant isolates and resistance genes were detected from15sample sits in urban sewage, whichprovide a theoretical basis for the scientific understanding of the relationship between urbansewage and ecological public healthy safety. |
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