Bioremediation Of Petroleum-contaminated Saline Soil With Arbuscular Mycorrhizal Fungi, Plant And Nitrogen

As oil exploration and the increase of usage, followed by a large amount of oil and itsproducts into the environment, resulting in the pollution of the environment in theprocess of mining, transportation, processing and using, harm to life on earth. Sincethe beginning of this century, bioremediation has become the main technology tosolve the oil pollution problems, which combined restoration is more popular than thesingle. In order to solve the petroleum pollution in Gudong of Dongying, I’m basedon the theory of the pollution ecology, for the research of petroleum pollutionremediation in laboratory.Selection of Dongying local halophyte Suaeda glauca Bge and advantages ofarbuscular mycorrhizal fungi Glomus mosseae, to establish arbuscular mycorrhizalfungi, plant, nitrogen addition petroleum pollution remediation. Analysis of thechanges of different pot experiment before and after the changes and the remediationof petroleum degradation situation, oil composition of the microbial communitystructure. Experimental results have been obtained as follows:(1) After90days of AM fungi-plants-nitrogen joint repair Pot experiment, CK, S, N,N+S, M+S and N+M+S group of oil degradation rate increased to36.98、50.30、48.86、55.51、55.73、61.26%. The highest degradation rate is the S+N+M group,1.66times of the CK group. AM fungi-plants-nitrogen add to repair is a betterchoice for oil pollution.(2)Through soil physical and chemical properties found that pH from8.50to7.78-8.27at the end of soil pot experiments, that can be slowed down degradation ofoil polluted soil in Dongying area salinization; in a certain range, the increase ofmoisture content for oil degradation; TN is a significant factor in oil degradation. (3) Analysis of spectra and chromatography method to obtain the four fractions can befound by gas chromatography, resin and asphalting fractions of constant volume ratioincreased, total amount of saturated and aromatic hydrocarbon reduction ratiodecrease, S+N+M group of saturated hydrocarbon degradation rate was3.2timeshigher than CK group, that bioremediation degradation of oil the saturatedhydrocarbon and aromatic.(4) The change of soil Gudong area of bacteria, fungi and mycorrhizal fungi AMcommunity structure and pot experiment in the Dongying using PCR-DGGEtechnology, the AM mycorrhizal fungus Glomus mosseae as the dominant species insoil has always existed; soil sample oil degradation rate were higher in the bacteriumBacillus sp. in bacteria the whole community larger proportion. These two kinds ofbacteria could be potential bacteria caused the oil degradation, but whether it isfeasible to further isolation and identification.(5)It can be used as the preferred plant Suaeda salsa oil contaminated soil remediationDongying Gudong area, AM mycorrhizal fungi in plant nitrogen addition combinedremediation technology can be used as Dongying Gudong area of high concentrationof petroleum contaminated saline soil restoration reserve technology and applied.