Synthesis And Properties Characterization Of Naphthphthalimide-based Compounds

Fluorescent sensors in the detection and measurement of environmentally and biologically relevant important ionic species, particularly for heavy-and transition-metal cations, are widely investigated because of their simplicity and high sensitivity of response.1,8-Naphthalimide derivatives are well-known fluorescent chemosensors with high absorption coefficient, high fluorescence quantum yield and high photostability. However, the development of new fluorescent Hg2+selective sensors is still a tremendous challenge, particularly in aqueous solutions and biological environments. Therefore, there is an urgent need to develop chemical sensors that are capable of detecting Hg2+in environmental and biological samples at a physiological pH value.In this dissertation, ten1,8-Naphthalimide derivatives were designed and synthesized, The target compounds were characterized by HR-MS,1H NMR and13C NMR.Naphthalimide-based fluorescent probes L1and L2were designed and synthesized, and they showed high sensitivity and selectivity for Hg2+over the other metal ions in the phosphate buffer. The fluorescence of the compound L1and L2could be quenched by up to90%with the addition of Hg2+into the proton solution.At last, we study the cleavage activity of these ligands (L4-L10) on the pUC19DNA through the agarose gel electrophoresis. Besides, This paper research from buffer, concentration, pH and reaction time to select the optimum condition. In addition, we have investigated the reaction mechanism on the cleavage DNA.