| The tissue engineering technology of bone has significance meaning for the repair of defect skull, tissue scaffold is an important content in the bone tissue engineering, but there are no mature total solution for it at present. This article, basing on the understanding of the current situation of the domestic, studied this problem systematically in order to solve it.First, we scanned human skull sample by the micro-CT technology, getting the 2-Dimage of the skull, and accomplished the 3-D modeling to the natural microporous structure by applying reverse engineering technology; then, a parameter model that cavityand communicating tube were connected into microporous structure according to certain space structure was proposed through our analysis to the model, and then the model parameters were analyzed statistically.Secondly, we carried out the bionic design for the skull according to the statistical analysis result of the natural microporous structure, designed algorithm to generate point cloud matrix of the space structure nets and based-cavity and based-pipe, and realized assembling and synergy deformation of the cavity and the pipe; then the point cloud treatment and surface fitting were conducted, getting the model of bionic microporous structure, and then we prepared the negative mould of tissue engineering scaffolds of skull using rapid prototype technology.Finally, we produced the designed bionic stents using gel mould technology. Studied the influence of catalyst and dry way on the mass of ceramic body and then determined the process flow of slip casting and drying, at last, formulated sintering schedule as well as carried through sintering experiment by analyzing the TGA/DSC of resin and gel.The results show that the tissue engineering scaffolds prepared by this scheme, has similar space structure with natural skull, which laid the foundation for further optimizing tissue scaffold and promoting the development of bone tissue engineering technology. |
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