Effect Of Puccinia Striiformis F.sp.tritici On Wheat Leaf Protein And Contents Of Cd, Pb, Cu

Wheat stripe rust was one of the most major wheat disease caused by stripe rust(Puccinia striiformis f.s p.tritici) infection. The fungus diseases of stripe rust isstrongly epidemic and seriously harmful. Using comprehensive management ofresistant varieties of wheat to prevented disease was a safe and effective way. Modernbreeders were facing with the issues that the physiological mutant variations of wheatstripe rust fungus resulted in the loss of varieties resistant, and the breeding speed ofnew resistant varieties was too slow. Before and after inoculation, the change of leafprotein expression in different resistant varieties and the content of the Cd, Pb, Cuelements were analyzed. We want to found the interaction of mechanism betweenwheat and wheat stripe rust fungus. The main results were as follows:1. Using the method of2-DE and MALDI-TOF/TOF MS detected15differentially expressed proteins from the three wheat varieties, including: light-harvesting complex I, the partial chloroplast; chloroplast light-harvesting chlorophylla/b binding protein; chlorophyll a/b-binding protein WCAB of precursor; germin-likeprotein; ribulose bisphosphate carboxylase; oxygen-evolving complex precursor; actin;peptidyl-prolyl cis-trans isomerase and a number of predicted proteins.2. There are7differential proteins in Mingxian169,6differential proteins inLantian15, and3differential proteins in Tian9524. Which indicated that with theincreasing of wheat stripe rust resistance, the impact of protein in turn weakened inMingxian169, Lantian15and Tian9524, showed that with the enhancement ofwheat’s resistance and the function of the resistance mechanism, the impact of plantwere reduced.3. Using the method of ICP-AES measured the element content of cadmium,lead, copper in leaves, stalks and roots of a susceptible variety to wheat stripe rust ofMingxian169, a resistant variety to wheat stripe rust of Lantian15and immunevarieties of Tian9524. The results showed that the contents of Cd, Cu in three varietiesof wheat which was not inoculated the bacteria on the leaves in different periods were not significantly changed, while the content of Pb with the passage of time took on agradually rising trend. After the inoculated, the content of Cu in three varietiespresented a trend of at first rising，then falling, and in the end steady. The trend ofMinxian169showed more positive, the Pb presented the trend that during the initialtime Pb was decreasing, and then increased. Cd in Minxian169was continuouslyrising, in Lantian15Cd first falled, after6d Cd increased; in Tian9524in the earlydays Cd had no change, but after6d it slowly rised. The content of Cd, Pb, Cu inleaves of Minxian169ranked first, followed by Lantian15and Tian9524. Cd inTian9524was much lower than the other two varieties. In the stem, after the inoculatthe content of Cd, Pb, Cu was rising; Cd, Pb in Lantian15falled, but Cu rised; Pb, Cuin Tian9524rised, but Cd significantly decreased. In roots, after the inoculat, thecontent of Minxian169and Tian9524were rising, the trend of rising in Minxian169was more significant, but in Tian9524, only Cu was slightly rising, the other elementswere decresing. The results showed that the stripe rust fungus infection had certaininfluence on the the Cd, Pb, Cu of host, and the changes of the content of Cd, Pb, Cuin wheat had a direct relationship with the ability of stripe rust disease resistance.