Establishment Of Rapid Multiplication System For Cremastra Appendiculata (D. DON)Makino

Cremastra appendiculata (D. Don) Makino has good effect on detumescence, peptization and detoxification as a dry pseudobulb. After years of over-exploitation, this wild medicinal vegetation is now unusual rare. Resent reserch has indicated that tissue culture is an useful way to obtain large number of good plantlet. While division propagation and seed production has shortcoming of long culture cycle and low efficiency, tissue bud propagation has advantages of short culture cycle and high efficiency. In view of the above backgrounds, it was significant to make factor analysis on tissue bud propagation, so as to develop an effective way to establish multiplicative system of culturing the C. appendiculata.In this paper, technique of tissue bud propagation was introduced to implement multiplicative system for culturing C. appendiculata, the following work had been done:First, explant of Iphigenia indica was cultured to provide rough materials for establishing the multiplicative system. Second, optimum dispensing of the culture medium was developped to establish the multiplicative system of culturing the C. appendiculata. Third, microstructure of the explant was observed to analyzed the morphogenetic movement of cells and their accumulation of substances. Fourth, outdoor culturing was explored to take preparation for industrial culturing.Results indicated that, the optimum explant for establishing multiplicative system was tawny bud and flavovirens bud; the optimum dispensing of the culture medium was1/4MS medium with0.1mg-L-1NAA,2.0mg-L-1BA,3%sucrose,0.75%active carbon,0.55%agar, with pH of5.8, where the tissue bud has the fastest growth rate of51.03%and weighting rate of52.49%during45days. This reserch has resolved problems of artificial propagation for C. appendiculata, which may lay a good foundation for application of the medicinal resource.