Study On The Genetic Diversity Of Quercus Liaotungensis Natural Population Inshanxi Province

Quercus liaotungensis is one of the most important forest resources in Shanxi province.However,the area of the Q. liaotungensis gradually reduced due to the artificial damage in recent years. So,the effective protection and exploitation is being very important. And the research of genetic diversity is the important part of conservation biology, so the paper analyse the Q.liaotungensis genetic diversity of Shanxi province, expecting to offer genetic basis for the protection. I selected9natural populations and used the SSR molecular markers to research the Q.liaotungensis natural populations, that included genetic diversity, genetic differentiation, genetic distance, cluster analysis and so on. The follows are the results:(1) Optimized the response program and system of SSR, and selected11primers that are suitable for the research of Q.liaotungensis genetic diversity.(2)The Q.liaotungensis populations in Shanxi have the high level of genetic diversity. The11primers got113bands,and the average number of alleles is10.2727,the effective numbers of alleles is5.1859, the Shannon diversity index is1.7543,the average expected heterozygosity was0.7538, Nei diversity index is0.7521. And,the population of Lingkong mountain has the highest level in genetic diversity, the population of Luya mountain has the lowest level in genetic diversity.(3) The heterozygote ratio of Q.liaotungensis populations is less than expectations,that means the number of the heterozygote is shortage.(4) The genetic differentiation of Q.liaotungensis populations is much higher, about95.99%of the genetic differentiation occurs in the inside, only4.01%of the genetic differentiation is happened between the populations. (5) In the geographic range of the research, the genetic distance and geographic distance present positive correlation, but when the geographic distance down to234.955km, the positive relationship between the genetic distance and geographic distance will disappear.(6) The Q.liaotungensis populations deviate from the Hardy-Weinberg balance.