Sex Control Of Bovine Embryos In Vitro By Using SRY Antibody

With the development of the mechanism of sex determination, the control ofmammalian sex has been a research hotspot for the recent years, sex controltechnology is also related to the cytology and molecular biology techniques. On theone hand，sex control of animals can give full play to the effectiveness of sex-limitedtrait, saving production cost. On the other hand,it can also reduce the occurrence ofsex-linked genetic disease, to reduce the losses of livestock industry. Sex-determiningRegion on the Y-Chromosome (SRY) is the key gene for sex determination anddifferentiation, which is the mammalian testis-determining factor, plays a vital role forthe decision of the male sex, that is the theoretical basis which most sex controltechniques rely on.In this study, different concentrations of SRY antibodies were used to deal withcattle semen which was used for in vitro fertilization, and then identified the sex ofthe embryos.The influence of using SRY antibodies for the sex of in vitro bovineembryos was known, the theoretical basis of the mechanism of sex determinationwas enriched.The study also had some value for livestock industry.The main resultsare as follows:1.Multiplex PCR with two pairs of primers was established to identify the sex ofbovine embryos. To verify the method,50bovine oocytes were used in PCR.Theaccuracy rate is100%.2.The culture system of bovine sex-control embryos with SRY antibody wasestablished.After matured the bovine oocytes in vitro,fertilizated with the semenwhich was handled by SRY antibody.190embtyos were obtained which had morethan4cells. To identify the sex of sex-control and normal embryos with multiplexPCR used two pairs of primers.When the concentration of antibody were0μg/L,200μg/L,400μg/L,the proportion of female embryos were56%（27/48）,75%（56/75）,82%（55/67）.The differences between proporation of female embryos whichobtained by antibody treatment or not were extremely significant（P＜0.01）.The proportion of female embryos was no significant difference when the concentrationsof SRY antibody were200μg/L,400μg/L(P>0.05).There was no significant effecton fertilization rate and the development of early embryos when used differentconcenrtations of SRY antibodies。In summary, the SRY antibody method can significantly improve the femaleproportion of of bovine embryos in vitro,which is an effective sex control method.