Analysis And Identiifcation Of Cotton Salt Induced Protein

Concomitant with the constant growth of population and the decrease of arable land, thecontradiction between cotton and farmland is becoming increasingly serious, thus fostering salttolerance cotton varieties for the maximum exploitation of saline-alkali land is not only the inevitabletrend of cotton cultivation but also the need of sustainable development of agriculture. At present,however, salt tolerance materials for practical use is rare, therefore conducting cotton salt tolerancestudy at molecular level is of great significance to enhance salt tolerance in cotton. The salt-tolerantmaterials Zhong07and salt-sensitive materials Zhongs9612were chosen for experimental material.After stressed by0.4%NaCl for0h,24h,72h,120h and168h at trefoil stage, total proteins ofsalt-tolerance Zhong07and salt-sensitive Zhongs9612were extracted using TCA-acetone precipitationmethod. Subsequently, followed by SDS-PAGE analysis, the protein profile between0h and24hpresented significantly difference. Therefore, the two time points were chosen for furthertwo-dimensional electrophoresis; salt tolerance related proteins were further screened using2Delectrophoresis, after24h salt stress,22protein spots showed significant changes, among which16spots were unregulated and6spots were down regulated; by mass spectrum analysis,14differentiallyexpressed proteins were successfully identified, including RuBisCO carboxylase/oxygenase activasealpha2, RuBisCO carboxylase/oxygenase large subunit, Unnamed protein product, OEE2RuBisCO large subunit-binding protein subunit alpha, Luminal binding protein, Heat shock protein70,PR protein class10, Quinone oxidoreductase-like protein, S-adenosylmethionine synthetase, Enolaseand RuBisCO large subunit-binding protein subunit beta, and bioinformatics analysis revealed that theywere involved in photosynthesis, energy metabolism, defense mechanisms, respectively; consequently,these findings lay a solid foundation to increase our understanding of salt tolerance mechanism in cottonand provide new insight into salt tolerance study in the future; simultaneously, to validate whether therewas consistency between transcription level and post-translation level, qRT-PCR analysis wasperformed,,indicating changes at protein level and mRNA level had a certain correlation,but it merelypartially agreed with each other.Based on the above research findings, we had a basic understanding of salt tolerance mechanism atproteomic level, and we found that the expression level of mRNA was not always consistent withtranslation of protein. This entailed related experiments such as western blotting to deepen ourunderstanding of salt tolerance in cotton and provide the foundation for the construction of regulationnetwork of salt tolerance in cotton.