Development And Validation Of A Monoclonal Antibody Trapping NSP-3ABC Blocking ELISA For Differenitation Of Foot-and-mouth Disease Virus Infected From Vaccinated Animals

The monoclonal antibody (mAb) is considered to be the best molecular probes, and non-structural protein (NSP)3ABC antibody is considered to be the most reliable indicator of present or past infection with foot-and-mouth disease virus (FMDV) in vaccinated animals.In this report, a non-structural proteins (NSP)3B monoclonal antibody(mAb) trapping3ABC-blocking ELISA was developed to detect FMDV NSP3ABC antibody. The histidine mAb and NSP3B mAb were used as capture antibody and detection antibody respectively, and purified His-tagged3ABC fusion protein were used as antigen.With statistics, the critical value0.46was defined as blocking rate after hundreds sera samples of cattle, sheep and pigs were detected. According to this criteria, the sensitivity of the positive serum>99.2%, the specificity of the negative serum>85.4%.This method not only has a higher coincident rate compared with Ceditest NS ELISA kits, but also has a highter positive detection rate.