Anlysis Of SSR Marker In Cabbage Germplasm Genetic Diversity And Purity Indentification Of F1

Through the investigation of Cabbage biological character and SSR molecular marker,the genetic diversity of113cabbage accessions were revealed. In addition, the author carriedout the research about the consistency of SSR molecular marker technique with fieldmorphological observation of hybrids purity in cabbage.The main results are as follow:1.31pairs of primers were selected for PCR amplification of the113samples of cabbage.It generated a total of212clear and repetitive bands, the average number are7.07. There are203polymorphic bands,9non-polymorphic bands, and the polymorphism rate was95.8%.2. It was obtained through cluster analysis: genetic similarity coefficients of cabbageranging from0.56to1.00. It was classified as two groups with the similarity coefficient of0.605, and eight groups with the similarity coefficient of0.66. Most of the domestic varietieshave small genetic distance, and have a closer relationship. And the genetic distance offoreign varieties is relatively large, the genetic relationship is far. It provides theoreticalfoundation for parent’s selection and new variety breeding in the future.3. This study investigated eight field morphological indexes of113cabbage material. Itwas obtained through cluster analysis: genetic similarity coefficients of cabbage ranging from0.70to1.00, and113cabbage materials were classified as five groups with the similaritycoefficient of0.732. The analysis showed that accessions in1,2groups have good consistency,but those accessions in3,4,5groups have small consistency, indicated that cabbagegermplasm accessions in3,4,5groups have relatively great difference.4. Primer SQ1019screening from SSR molecular markers can be used to rapid purityidentification of “Qin Gan50”.5. The purity of “Qin Gan50” was96.3%by SSR molecular marker. Field identificationof “Qin Gan50” with its corresponding plant of SSR was96.0%, the results indicated thatthey were the same for hybrid identification between SSR approach and morphologicalassessment, the consistency rate of them reached99.7%. SSR molecular marker technique is aquick and accurate method to identify seed purity...