| Northeast forest frog (Rana dybowskii) mainly lives in Chinese sub-arctic northeastern provinces and Inner Mongolia. Rana dybowskii have to face the severe cold of threats and pressure of survive in the winter. Thus as northern cold amphibian species, the research of cold tolerance characteristic is highly valuable; In addition, they are important economic animals, the study of overwintering safely is real economic significance. We determined the impact of low temperature on the glucose, liver glycogen, muscle glycogen content of Rana dybowskii and also tested the impact of low temperature on the activities of GP, PEPCK of Rana dybowskii.We also tested the blood glucose level change rule of the frog which accepted different concentration glucose at4℃and-1℃to investigate the relationship between the change discipline of biochemical substances in body and cold resistance, providing valuable theoretical basis and experimental data for amphibian cryobiology.The result:the northeast forest frog’s survive ratio is100%at4℃、0℃、-1℃after low temperature intimidate, survive ratio fell to0at-2℃and0at-3℃. Results showed that glucose content in4℃,0℃and-1℃groups were significantly increased (P<0.01) compared with those in23℃group, glycogen, muscle glycogen content in4℃,0℃and-1℃groups were significantly decreased (P<0.01) compared with those in23℃group. Liver and muscle GP activities in0℃and-1℃groups were observably increased (P<0.01) compared with those in23℃group. Liver and kidney PEPCK activities in0℃and-1℃groups were observably higher (P<0.01) than those in23℃group. |
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