Differences Protemics And Transcriptomics Analysis Of A Gossypium. Hirsutum Virescent Mutant By Space Mutantion

The cotton virescent mutants had very important value in practical. On one hand it could be used as a natural mark for breeding to reduce time of the hybrid breeding and process of the seed identification, on the other hand they were ideal model systems to study chloroplast biogenesis and the nature of communication between the nucleus-cytoplasm, chloroplast and mitochondrion. In order to obtain the molecular mechanism of the virescent mutant in cotton were of great theoretical and practical significance.In this research CCRI58and its space mutate CCRI58vsp were used as experiment materials. The virescent phenomenon of the CCRI58vsp expressed when the first true leaf emerged; it was not ended until the flowering period. The true leaf was pale yellow when they came out, after3days the virescent became most severely,5days later the leaf went to restore gradually, then7days later some part of leaf came back green,9days later the color of the leaf became green as the same as the wild type leaf. The experiment was based on both leaves from the first euphylla to the fifth in virescent cotton and wild type, two-dimensional electrophoresis (2-DE) were used to look for the total proteins of the second leaf from the top, and then detected and identified differential proteins which may concern with etiolation. Besides isolated the differentially expressed genes of the CCRI58vsp and CCRI58using SSH and cDNA microarray, for help us understand the molecular mechanism of the virescent mutant. The results were as follows:1. We use the total leaf proteins of the second leaf from the top of the CCRI58vsp and CCRI58(the most severely etiolation) by2-DE, subsequently, professional2D imagermaster7.0software were applied for analyzing,45differentially proteins between the CCRI58and CCRI58vsp.18proteins were identified at last. There proteins involved in photosynthesis, signal transduction, translation, metabolic and so on. Though these proteins, they indicated cell metabolic disorder, chloroplast development abnormal, restrained of the chlorophyll biosynthesis, the molecular mechanism of the virescent mutant was complex, the process of the virescent may be complex system by many proteins taking part in.2. We amplified and purified the2459clones which came from previously four SSH library to form chip, used both of the CCIR58vsp second leaf, the second and fifth leaf of the CCIR58vsp to hydride from chips. In chip V2vs V5,115genes were up-regulation,119genes were down-regulation. In the chip V2vs V5,95genes were up-regulation,102genes were down-regulation. The functional classification of there unigenes was carried out based on the MIPS functional catalogue. Most of them assigned to subcellular localization (ribosome and chloroplast), structural or catalytic, transcription, metabolism and so on. These genes involved in ribosome, photosynthesis, oxidative phosphorylation, purine and pyrimidine metabolism and chlorophyll biosynthesis. We found many of the same genes in these two different chips, the same genes may play a key role in the molecular mechanism of the virescent mutant (such as ribosome protein, thylakoid membrane proteins). In addition genes with no match the database or unknown played a certain function.