Genetic Diversity And Fingerprinting Of Hybrid Parents In Brassica Napus L.

These years, rapeseed had got rapid development, and China had become the largest country in the world which group rape. Heterosis is an important way to increase crop yields. while the genetic differences between the parents is to generate heterosis. Comprehensive and systematic understanding of the genetic differences, genetic distance among the parents were very important, it was useful to understand the genetic background of the parents, then maybe helpful for selecting parents and forcasting prospect of breeding. For commercial seeds, hybrid seed has high commercial value and it palys importance role in seed trade. In seed market, some people sell false or inferior seeds, and some of them gain hybrid parents illegally to Pursuit of profit maximization. It severely damage the lawful rights and benefits of the breeders or legal companies. Therefore, how to protect hybrid parents and identify hybrid purity becomes more and more important in hybrid production and commercialization.The genetic diversityof92hybrid parentsof Brassic napus.L was analysised by SSR markers, these SSR datas were used to analysis the genetic distances and their relationship these results offered theoretic reference for breeding. Meanwhile, the DNA fingerprint were constructed for24hybrid core parents by the SSR datas. The DNA fingerprint may identified the varieties of authenticity and purity, to determine the genetic relationship of the species, it alsocan be used for the registration of new varieties and species of intellectual property protection.The followingis the main results:1. Three male sterile lines and three restorer lines,randomly selected from the92tested materials, formed a small sample to screen the900primers in our lab.Excluding non-amplified primers and primers with poor specificity,249SSR primers were chosen.2.Eight representative materials randomly selected were amplified on249SSR primers again Excluding non-amplified primers and primers with poor specificity,68SSR primers were chosen.3.68primers were amplified in92Brassic napus.L.258bands were polymorphic bands of280amplified bands.The polymorphism rate was92.1%.4.258polymorphic bands were used for clustering analysis with UPGMA.92accessions of Brassic napus. L. were classified into8groups by the threshold value of 0.685. All the sterile lines were clustered into Group Ⅰ and restorer lines were clustered into Group Ⅰ,Ⅲ,Ⅳ,Ⅵ and Ⅶ, except P58outside,8-8110,8X-310and8X179-180. These results indicated that restorer lines had more genetic diversity than sterile lines and genetic distance between the sterile lines and the restorer lines was larger than that among the sterile lines or among the restorer lines.5.DNA fingerprints were constructed for24Brassica napus L. core parents with6SSR primers,and the6SSR primers could distinguish all of the24Brassica napus L. core parents.