Isolation, Identification And Pathogenicity Of Bacterial Diseases In Pinellia Ternate

Pinellia ternata(Thunb) Breit is a perennial herb belonging to the family Araceae.lts dried tubers are common traditional Chinese medicinal materials.With wild resource of Pinellia ternata was less and less by people used,to solve the contradiction between supply and demand to protect the wild resources.Our country from20century began aitificial planting wild Pinellia ternata and the cultivation technology research.Soft rot disease of Pinellia ternata is Pectobacterium carotovora cause.The disease occurs common, lead to Pinellia ternata production declined serious and threatens the large-scale production.This thesis aims to the main diseases of P.ternata--Soft rot diseases,focus in the separation of the bacteria, screening and identification,At the same time also on the pathogenitity conditions and law to research.In order to lay the foundation of this disease control. The main research results are as follows:1Separated17strains from the rot organization. According to the requirements of Koch’s rule inoculated back to the health of P.ternata,found three strains isolated make the health of P.ternata arise disease and appear same symptoms with acquisition in disease field,they name is JS4,JS8,JS13.Will this3kinds of bacteria vaccination in Chinese cabbage, carrots and cucumber,appear the same soft rot symptoms.To illustrate the three kinds of bacteria is broad spectrum of pathogenic bacteria,Consistent with other research reports.2Completed the molecular identification to the JS4,JS8and JS13, found the JS4and JS8is the same kinds of bacteria,belongs to Pectobacterium carotovorum subsp.carotovorum. JS13belongs to klebsiella oxytoca coli, is a new pathogenic bacteria of Pinellia ternata and have been no reports so far. At the same time, operated identification of character of the physiological and biochemical to JS8and JS4, its results and molecular appraisal result is consistent.3In the test of conditions of occur soft rot disease and pathogenesis regularity,we found:under the conditions of high temperature high and moisture diseases occured seriously The disease occurs the optimum temperature is25-30℃.Humidity is high, the more disease occurs obviously.In the pathogenic way research, found the disease incidence between the health and the wound of P.ternata is significant differences,Explain the wound is one of the main ways for invading germs.4In the experiments of different agentia to the inhibition ratio of pathogenic bacteria,72%Agricultural streptomycin and6%kasugamycin to P.ternata soft rot virulence is the highest.